绵羊痘病毒和山羊痘病毒通用RPA检测方法的特异性试验  

Specificity Test of the Universal RPA Detection Method for Sheep Pox Virus and Goat Pox Virus

作　　者：豆玲[1] 周峰[1] 高军军 王雪莹 王志宇 康文彪[1] DOU Ling;ZHOU Feng;GAO Jun-jun;WANG Xue-ying;WANG Zhi-yu;KANG Wen-biao(Animal Disease Prevention and Control Center,Lanzhou,Gansu 730046,China;Yingke Xinchuang(Suzhou)Biotechnology Co.,Ltd.)

机构地区：[1]甘肃省动物疫病预防控制中心,甘肃兰州730046 [2]英科新创(苏州)生物科技有限公司

出　　处：《畜牧兽医杂志》2023年第3期21-22,共2页Journal of Animal Science and Veterinary Medicine

基　　金：甘肃省农业农村厅科技项目(GNKJ-2021-24)。

摘　　要：应用我们建立的绵羊痘病毒和山羊痘病毒通用RPA检测方法,通过对口蹄疫病毒、传染性脓疱皮炎病毒、鸡痘病毒、绵羊痘病毒、羊痘疫苗和羊痘重组质粒样本的检测,验证绵羊痘病毒和山羊痘病毒通用RPA检测方法对绵羊痘/山羊痘病毒检测的特异性。结果显示该方法对羊痘疫苗、羊痘重组质粒、绵羊痘病毒核酸和山羊痘病毒核酸阳性样品能够在试纸条上显示出阳性条带,而对其他样品不显示阳性条带。表明该方法具有良好的特异性,在绵羊痘/山羊痘病毒检测中应用前景广阔。In this study,the previously established general RPA detection method for sheep pox virus and goat pox virus was used to detect foot-and-mouth disease virus,infectious pustular dermatitis virus,fowl pox virus,sheep pox virus,sheep pox vaccine and sheep pox recombinant plasmid samples.Based on this,we verified the specificity of the general RPA detection method for sheep pox virus and goat pox virus for the detection of sheep pox virus and goat pox virus.The results show that the method can show positive bands on the test strip for the positive samples of sheep pox vaccine,sheep pox recombinant plasmid,sheep pox virus nucleic acid and goat pox virus nucleic acid,but does not show positive bands for other samples.This shows that the method has good specificity and has broad prospects in the detection of sheep poxvirus and goat poxvirus.

关 键 词：重组酶聚合酶扩增技术(RPA) 山羊痘病毒 试纸条 绵羊痘病毒 

分 类 号：S852.654[农业科学—基础兽医学]