应用TRBC1鉴别T细胞克隆的研究  

Identification of T cell clones by TRBC1

作　　者：马耀坤 郑金娥[3,4] 李小青 宋亮亮[1] 刘伟 李娟 商芳影[1] 张雨中 杜雯[3,4] MA Yaokun;ZHENG Jine;LI Xiaoqing;SONG Langlang;LIU Wei;LI Juan;SHANG Fangying;ZHANG Yuzhong;DU Wen(Flow Cytometry Laboratory,Wuhan Kindstar Medical Laboratory Co.,Ltd,Wuhan,430074,China;Taikang Medical Schoolof Wuhan University;Department of Stem Cell,Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology;Biological Targeted Therapy,Key Laboratory in Hubei)

机构地区：[1]武汉康圣达医学检验所有限公司流式细胞实验室,武汉430074 [2]武汉大学泰康医学院(基础医学院) [3]华中科技大学同济医学院附属协和医院干细胞科 [4]生物靶向治疗研究湖北省重点实验室

出　　处：《临床血液学杂志》2023年第4期260-264,共5页Journal of Clinical Hematology

摘　　要：目的:研究使用流式细胞术T细胞抗原受体β恒定区1(TRBC1)基因单克隆抗体鉴别T细胞克隆方法的可靠性。方法:回顾2021年6月—2022年2月的78例样本,分别使用流式细胞术TRBC1标记法、T细胞抗原受体可变区β链(TCRVβ)法及分子T细胞抗原受体(TCR)重排法,通过几种方法比较得到TRBC1判断T细胞克隆的可行性。并比较TRBC1法在不同疾病中的表达及与其他相关CD分子的共表达情况是否存在差异。结果:使用TRBC1法的表达率<15%和>85%作为克隆阳性判断标准,与TCRVβ法及TCR重排法判断克隆性比较,差异无统计学意义(P=1.000,0.250);且其kappa值分别为0.871和0.873,为高度吻合性。在不同疾病T细胞克隆判断中,除TCRγ/δ淋巴瘤外,在其他疾病T细胞克隆判断上均差异无统计学意义(P>0.05)。TRBC1的表达率与CD2、CD3、CD4、CD5、CD7、CD8、TCRα/β表达均无相关性(|r|<0.3),可作为独立的判断因素。结论:使用TRBC1法同其他方法比较具有较高的一致性,方法可靠;且其经济高效,试验方法简单,值得临床作为T系克隆的判断方法进行推广。Objective To investigate the reliability of flow cytometry TRBC1 monoclonal antibody in identifying T cell clones.Methods Reviewing seventy-eight samples from June 2021 to February 2022,TRBC1 method,TCRVβmethod and TCR rearrangement method were respectively used to compare the feasibility of TRBC1 to identify T cell clones.The expression of TRBC1 with different CD molecules and in different diseases was compared.Results When TRBC1 expression rate in<15%and>85%was used as the criterion for determining clonal positive,compared with TCRVβmethod and TCR rearrangement method,the P values were 1.000 and 0.250 respectively,and there was no significant difference.The kappa values were 0.871 and 0.873,respectively,indicating high consistency.There was no significant difference in the clonal judgment of T cells in different diseases except TCRγ/δlymphoma(P>0.05).CD2,CD3,CD4,CD5,CD7,CD8,TCRα/βexpression and TRBC1 expression had no correlation(|r|<0.3),which could be used as an independent diagnostic factor.Conclusion Compared with other methods,TRBC1 method may hanve a high consistency.It may be economical,efficient and simple.It is worthy of clinical application as a judgment method of T-line cloning in clinical practice.

关 键 词：T细胞克隆 流式细胞术 TRBC1 单克隆抗体 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]