蠲痹颗粒醇提物抑制Th17细胞表达治疗类风湿关节炎作用机制研究  

Study on Mechanism of Alcohol Extract of Juanbi Granules in Inhibiting Th17 Cell Expression in the Treatment of Rheumatoid Arthritis

作　　者：吴招 邹楠婷 顾茜兰 杨海浩 应赛 李小丝 祁燕 彭江云 万春平 WU Zhao;ZOU Nanting;GU Qianlan;YANG Haihao;YING Sai;LI Xiaosi;QI Yan;PENG Jiangyun;WAN Chunping

机构地区：[1]云南中医药大学,云南商昆明650500

出　　处：《新中医》2023年第9期7-11,共5页New Chinese Medicine

基　　金：国家自然科学基金项目(81360543);云南省卫生科技计划项目(2017NS166,2016NS2177)。

摘　　要：目的:研究蠲痹颗粒醇提物抑制致病性Th17细胞表达治疗类风湿关节炎(RA)的作用机理,阐述扶阳学派及吴生元教授运用扶阳理论治疗RA的分子机制。方法:构建牛Ⅱ型胶原诱导的DBA/1小鼠关节炎(CIA)模型,模型小鼠按随机数字表法分为CIA模型组(Model组)和蠲痹颗粒醇提物干预组(JBKL组)各10只。苏木精-伊红(HE)染色检测CIA小鼠关节炎病理损伤程度;流式细胞术检测Th17、Treg细胞表达水平;荧光定量PCR法检测Th17细胞相关基因的表达。结果:与Model组比较,JBKL组小鼠关节组织学评分降低(P<0.05)。与Model组比较,JBKL组Foxp3^(+)CD4^(+)T(调节性T细胞)表达水平上调(P<0.05)。与Model组比较,JBKL组小鼠脾淋巴细胞IL-17^(+)CD4^(+)比例降低(P<0.05),Treg/Th17比值增加(P<0.05)。JBKL组小鼠脾淋巴细胞IL-17A、IL-23p19、IL-21、IFN-γ的mRNA表达水平低于Model组,Th17特异转录因子RORγt的转录水平低于Model组(P<0.05)。结论:蠲痹颗粒醇提物可能抑制Th17细胞相关基因表达来抑制Th17细胞分化,上调具有负调控作用的Treg细胞表达,从而介导对RA的治疗作用。该研究为温阳散寒除湿法治疗寒湿痹阻型RA提供科学基础,丰富和发展了中医治疗痹证理论的科学内涵。Objective:To study the mechanism of alcohol extract of Juanbi Granules in inhibiting pathogenic Th17 cell expression to treat rheumatoid arthritis(RA),and expound the molecular mechanism of yang-reinforcing theory in treating RA by Yang-Reinforcing School's Prof.WU Shengyuan.Methods:The models of DBA/1 mice with typeⅡcollagen-induced arthritis(CIA)were established,and the model mice were divided into the CIA model group(Model group)and the alcohol extract of Juanbi Granules intervention group(JBKL group),with 10 mice in each group.The pathological injury of arthritis in CIA mice was detected by hematoxylin-eosin(HE)staining;the expression levels of Th17 cell and Treg cell were detected by flow cytometry;the expressions of genes related to Th17 cell were detected by quantitative real-time PCR.Results:Comparing with those in the Model group,the scores of joint histology in the JBKL group were decreased(P<0.05).Comparing with that in the Model group,the Foxp3^(^(+))CD4^(^(+))T(Treg cell)expression level was up-regulated(P<0.05).Comparing with those in the Model group,the IL-17^(^(+))CD4^(^(+))proportion in lymphocytes of the spleen in the JBKI group was decreased(P<0.05),and the Treg/Th17 ratio was increased(P<0.05).The mRNA expression levels of IL-17A,IL-23p19,IL-21 and IFN-γin the JBKL group were lower than those in the Model group,and the transcriptional level of Th17 specific transcription factor RORγt was lower than that in the Model group(P<0.05).Conclusion:The alcohol extract of Juanbi Granules may inhibit Th17 cell differentiation by inhibiting the expressions of genes related to Th17 cell and up-regulate the Treg cell expression with negative regulation effect,which thus mediates the curative effect of Juanbi Granules on RA.This study provides scientific basis for treating RA with colddamp obstruction syndrome by the method of warming yang,dissipating cold and eliminating dampness,and enriches and develops the scientific connotations of theory of traditional Chinese medicine for the treatment of impedim

关 键 词：类风湿关节炎 温阳散寒除湿法 蠲痹颗粒醇提物 Th17细胞 TREG细胞 RORΓT 

分 类 号：R285.5[医药卫生—中药学]