巴西苏木素通过激活Wnt/β-catenin通路调控卵巢癌SKOV3细胞增殖、凋亡、迁移和侵袭的研究  

Brazilin regulates the proliferation,apoptosis,migration and invasion of ovarian cancer SKOV3 cells by activating Wnt/β-catenin pathway

作　　者：贾晓晴 佟晓哲 JIA Xiao-qing;TONG Xiao-zhe(Acupuncture and Moxibustion Department of Traditiona Clhinese Medicine,The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China)

机构地区：[1]锦州医科大学附属第一医院中医针灸科,辽宁锦州121000

出　　处：《中国临床药理学杂志》2023年第9期1237-1241,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81473611);辽宁省科技计划面上基金资助项目(2015020343)。

摘　　要：目的探讨巴西苏木素对人卵巢癌SKOV3细胞增殖、凋亡、迁移和侵袭的影响及其作用机制。方法体外培养人卵巢癌SKOV3细胞,随机分为空白组及低、中、高剂量实验组和联合组。低、中、高剂量实验组分别用20、30和40μmol·L-1巴西苏木素处理细胞,联合组用40μmol·L-1巴西苏木素和10 ng·mL-1DKK1共同处理细胞,空白组给予常规培养。用流式细胞术检测细胞的凋亡率,用细胞划痕、Transwell小室实验分别检测细胞的迁移、侵袭能力,用蛋白质印迹法检测B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、E-钙黏蛋白(E-cadherin)蛋白的表达水平。结果中、高剂量实验组和空白组的凋亡率分别为(15.24±1.20)%、(25.13±1.46)%和(3.26±0.78)%,划痕愈合率分别为(20.55±3.16)%、(9.58±1.92)%和(45.76±2.24)%,侵袭细胞数分别为(100.00±4.00)、(44.66±10.44)和(207.66±15.11)个,Bax蛋白相对表达水平分别为0.78±0.03、1.32±0.09和0.25±0.11,Bcl-2蛋白相对表达水平分别为0.76±0.05、0.48±0.02和1.02±0.01,E-carderin蛋白相对表达水平分别为0.80±0.05、1.19±0.02和0.30±0.03。中、高剂量实验组的上述指标与空白组比较,差异均有统计学意义(均P<0.05)。结论巴西苏木素能够抑制SKOV3细胞增殖、迁移、侵袭和EMT进程,其机制可能与激活Wnt/β-catenin信号通路有关。Objective To investigate the effect and mechanism of Brazilin on proliferation,apoptosis,migration and invasion of SKOV3 cells in human ovarian cancer.Methods Human ovarian ccancer SKOV3 cells were cultured in vitro,and blank group,experimental-L,experimental-M,experimental-H groups and combined group were randomly set up.Experimental-L,-M,-H groups were treated with 20,30 and 40μmol·L^(-1)Brazilin,respectivley.In combined group,the cells were co-treated by 40μmol·L^(-1)Brazilin and 10 ng·mL^(-1)DKK1,and the blank group was cultured normally.Flow cytometry was used to measure the apoptosis rate of SKOV3 cells after administration of Brazilinadministration.Cell scratchesand Transwell chamber experiments were used to explore the effect of Brazilin administration on migration and invasion ability of SKOV3 cells.Western blot method was used to detect the expression levels of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax)and E-cadherin proteins.Results The apoptosis rates of experimental-M,-H groups and blank groupwere(15.24±1.20)%,(25.13±1.46)%and(3.26±0.78)%;the scratch healing rates were(20.55±3.16%)%,(9.58±1.92)%and(45.76±2.24)%;the number of invading cells was(100.00±4.00),(44.66±10.44)and(207.66±15.11);the relative expression levels of Bax protein were 0.78±0.03,1.32±0.09 and 0.25±0.11;the relative expression levels of Bcl-2 protein were 0.76±0.05,0.48±0.02 and 1.02±0.01;the relative expression levels of E-carderin protein were 0.80±0.05 and 1.19±0.02 and 0.30±0.03;the above indexes were statistically different between the experimental-M,-H groups and the blank group(all P<0.05).Conclusion Brazilin can inhibit the proliferation,migration,invasion and EMT process of SKOV3 cells,and its mechanism may be related to the activation of Wnt/β-catenin signaling pathway.

关 键 词：巴西苏木素 SKOV3 凋亡 上皮间质转化 WNT/Β-CATENIN 

分 类 号：R28[医药卫生—中药学]