木犀草素对大鼠肠系膜上动脉舒张作用的影响  

Effects of luteolin on the relaxation of superior mesenteric artery in rats

作　　者：李佳朋 梁晓莉 张湛睿[1] 贾敏[2] 郑梦迪 张彦[1] 侯颖 张寒[1] 陈玉龙[2] LI Jia-peng;LIANG Xiao Li;ZHANG zhan-rui;JIA Min;ZHENG Meng-di;ZHANG yan;HOU ying;ZHANG Han;CHENG Yu-long(School of Pharmacy,Xi'an Medical University,Xi'an 710021,Shaanxi Province,China;Institute of Basic Medicine and Translational Medicine,Xi'an Medical University,Xi'an 710021,Shaanxi Province,China;Pharmacy,FUping County Hospital,Weinan 711700,Shaanxi Province,China;Shaanxi Buchang Pharmaceutical Co.,LTD.,Xianyang 712000,Shaanxi Province,China)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]西安医学院基础医学与转化研究所,陕西西安710021 [3]富平县医院药房,陕西渭南711700 [4]陕西步长制药有限公司,陕西咸阳712000

出　　处：《中国临床药理学杂志》2023年第9期1257-1261,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家级大学生创新创业训练基金资助项目(S202011840022);陕西省科技厅自然科学基金资助项目(2022JQ-864);陕西省教育厅自然科学基金资助项目(21JK0883);陕西省科技创新基地-科技资源开放共享平台基金资助项目(2019PT-26);陕西高校青年创新团队基金资助项目(陕教[2019]90号)。

摘　　要：目的观察木犀草素对大鼠肠系膜上动脉的舒张作用并探讨其作用机制。方法采用离体血管环实验方法,1×10^(-6.1)、1×10^(-5.6)、1×10^(-5.1)、1×10^(-4.6)、1×10^(-4.1)、1×10^(-3.6)、1×10^(-3).1mol·L^(-1)木犀草素加药组为实验组,同时设立等体积累加生理盐水组为对照组。使用Myograph动态描记系统分别记录高K+、苯肾上腺素(PE)对大鼠离体血管环的收缩作用;观察不同浓度木犀草素对高K^(+)、PE预收缩血管环的舒张作用;同时采用多种工具药干预,探讨木犀草素舒张血管作用与内皮、钾通道及钙通道的相关性。结果木犀草素对高K+和PE预收缩的内皮完整的微动脉血管最大舒张率分别为(42.75±3.13)%和(82.64±1.80)%;其对去内皮微动脉血管环的最大舒张率为(94.52±0.76)%;在灌注压力存在的条件下,其对高K+和PE预收缩的微动脉舒张外径分别为(160.0±3.2)和(280.0±2.2)μm;当用浓度为1×10-4.6和1×10^(-4).1mol·L^(-1)的木犀草素孵育微动脉环后,PE累积添加浓度为1×10-5mol·L^(-1)时,PE最大收缩率降低,分别为(92.36±6.33)%和(63.76±12.79)%;实验组的上述数据与对照组比较,差异均有统计学意义(均P<0.05)。结论木犀草素对高K+和PE引起的血管收缩有浓度依赖性的舒张作用,其舒血管的机制与受体操纵性钙通道(ROCC)和电压依赖性钙通道(VDCC)有关。Objective To observe the relaxation effect of luteolin on superior mesenteric artery in rats and explore its mechanism.Methods The isolated vascular ring experiment method was used.The 1×10^(-6.1),1×10^(-5.6),1×10^(-5.1),1×10^(-4.6),1×10^(-4.1),1×10^(-3.6),1×10^(-3.1)mol L^(-1)luteolin group was the experimental group,and the equal volume of cumulative saline group was the control group.The contraction effects of high K+and phenylephrine(PE)on isolated rat vascular rings were recorded by Myograph dynamic recording system.To observe the relaxation effect of different concentrations of luteolin on high K+and PE pre-contracted vascular rings;at the same time,various instrumental drugs were used to explore the correlation between the vasodilation of luteolin and endothelium,potassium channels and calcium channels.Results The maximum vasodilation rates of luteolin on high K+and PE pre-contracted endothelial intact arterioles were(42.75±3.13)%and(82.64±1.80)%,respectively.The maximum relaxation rate of endothelium-denuded arteriolar rings was(94.52±0.76)%.In the presence of perfusion pressure,the outer diameters of arterioles precontracted with high K+and PEwere(160.0±3.2)and(280.0±2.2)μm,respectively.After incubation with luteolin at concentrations of 1×10^(-4.6)and 1×10^(-4.1)mol·L^(-1),the maximum contraction rate of PE decreased to(92.36±6.33)%and(63.76±12.79)%,respectively,when the cumulative concentration of PE was 1×10^(-5)mol·L^(-1).The above data in the experimental group were significantly different from those in the control group(all P<0.05).Conclusion Luteolin can significantly relax the superior mesenteric artery in rats,and it is more sensitive to the relax of deendothelial micro arterioles.Its mechanism is related to acceptor operant calcium channel(ROCC)and voltage dependent calcium channel(VDCC).

关 键 词：木犀草素 微动脉 受体操纵性钙通道 电压依赖性钙通道 外钙内流 内钙释放 

分 类 号：R28[医药卫生—中药学]