胆木注射液雾化吸入对小鼠安全性的影响  

The safety of aerosol inhalation of Danmu injection in mice

作　　者：郑雪健 苗佳宁[2] 刘波[2] 赵立[1] ZHENG Xue-jian;MIAO Jia-ning;LIU Bo;ZHAO Li(Department of Pulmonaryand Critical Care Medicine,Shengjing Hospital of China MedicalUniversity,Shenyangg 11000,LiaoningProvince,China;Medical Research Center of Shengjing Hospital,China Medical University,Key Laboratory of Research and Application of Animal Models for Enwironmental and Metabolic Diseases,Benxi 117000,Liaoning Province,China)

机构地区：[1]中国医科大学附属盛京医院呼吸与危重症学科,辽宁沈阳110000 [2]中国医科大学附属盛京医院实验中心、辽宁省环境与代谢疾病动物模型研究与应用重点实验室,辽宁本溪117000

出　　处：《中国临床药理学杂志》2023年第9期1267-1271,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82170047);“盛京学者”领军人才支持计划基金资助项目(M1145)。

摘　　要：目的探究胆木注射液雾化吸入给药对小鼠呼吸道及全身安全性的影响。方法将80只ICR小鼠随机分为对照组(等量0.9%NaCl)、低剂量实验组(1.56 mg·kg^(-1)胆木注射液)、中剂量实验组(2.50 mg·kg^(-1)胆木注射液)和高剂量实验组(5.00 mg·kg^(-1)胆木注射液),每组20只。各组小鼠均连续7 d雾化给药,qd,每次持续20 min。用多道生理记录仪检测末次雾化给药后的血氧饱和度;用酶联免疫吸附(ELISA)法检测血清白细胞介素-6(IL-6)水平;用二喹啉甲酸(BCA)法检测支气管肺泡灌洗液(BALF)中白蛋白的含量;用酶活性检测法检测乳酸脱氢酶(LDH)含量;用瑞氏吉姆萨染色法计数嗜酸性粒细胞占白细胞总数的百分比。结果对照组和低、中、高剂量实验组在末次雾化给药后2 h血氧饱和度分别为(76.00±4.64)%、(70.60±0.89)%、(77.00±3.54)%和(78.40±3.36)%;血清IL-6水平分别为(11.08±5.24)、(9.52±4.53)、(12.88±1.86)和(12.82±1.69)pg·mL^(-1);BALF中白蛋白含量分别为(0.12±0.37)、(0.16±0.30)、(0.13±0.50)和(0.13±0.16)mg·μL^(-1);LDH含量分别为(0.26±0.47)、(0.35±0.18)、(0.35±0.18)和(0.29±0.20)μmol·mL^(-1)。各剂量实验组上述指标与对照组比较,差异均无统计学意义(均P>0.05)。对照组和低、中、高剂量实验组嗜酸性粒细胞占比分别为(3.96±1.07)%、(2.31±1.16)%、(2.94±1.17)%和(3.08±1.13)%,低剂量实验组与对照组比较,差异有统计学意义(P<0.05)。结论胆木注射液雾化吸入对ICR小鼠具有良好的安全性。Objective To explore the effect of Danmu injection aerosol inhalation on respiratory tract and systemic safety in mice.Methods Eighty ICR mice were randomly divided into control group(equal volume of 0.9%NaCl),experimental-L group(1.56 mg·kg^(-1)Danmu injection),experimental-M group(2.50 mg·kg^(-1)Danmu injection)and experimental-H group(5.00 mg·kg^(-1)Danmu injection),with 20 in each group.The mice in each group were atomized for 7 consecutive days,quaque die(qd),for 20 minutes each time.The blood oxygen saturation after the last atomization was measured with a multi-channel physiological recorder;the level of serum interleukin 6(IL-6)was detected by enzyme-linked immunosorbent assay(ELISA);the content of albumin in bronchoalveolar lavage fluid(BALF)was measured by bicinchoninic acid(BCA);the content of lactate dehydrogenase(LDH)was detected by enzyme activity assay;the percentage of eosinophils in the total number of white blood cells was counted by Reich-Giemsa staining.Results The blood oxygen saturation of control group and experimental-L,-M,-H groups were(76.00±4.64)%,(70.60±0.89)%,(77.00±3.54)%,and(78.40±3.36)%,respectively at 2 hours after the last atomization;the serum IL-6 levels were(11.08±5.24),(9.52±4.53),(12.88±1.86)and(12.82±1.69)pg·mLstaining.Results The blood oxygen saturation of control group and experimental-L,-M,-H groups were(76.00±4.64)%,(70.60±0.89)%,(77.00±3.54)%,and(78.40±3.36)%,respectively at 2 hours after the last atomization;the serum IL-6 levels were(11.08±5.24),(9.52±4.53),(12.88±1.86)and(12.82±1.69)pg·mL^(-1)rerspectively;the levels of BALF albumin were(0.12±0.37),(0.16±0.30),(0.13±0.50)and(0.13±0.16)mg·μL^(-1);LDHwere(0.26±0.47),(0.35±0.18),(0.35±0.18)and(0.29±0.20)μmol·mL^(-1).There was no statistically significant difference between the above indexes of the experimental groups and the control group(all P>0.05).The percentage of eosinophils in the total number of white blood cells were(3.96±1.07)%,(2.31±1.16)%,(2.94±1.17)%and(3.08±1.13)%,respe

关 键 词：胆木注射液 雾化吸入 安全性 

分 类 号：R974[医药卫生—药品]