一种用于超低浓度黄曲霉毒素B1快速检测的生物传感器  

A Biosensor for Rapid Detection of Aflatoxin B1 at Ultralow Concentrations

作　　者：赵文辞 尤方朔 黄陈炜 汪涛 张鉴 戚昊琛 ZHAO Wenci;YOU Fangshuo;HUANG Chenwei;WANG Tao;ZHANG Jian;QI Haochen(School of Microelectronics,Hefei University of Technology,Hefei Anhui 230009,China;College of Electrical and Electronic Engineering,Wenzhou University,Wenzhou Zhejiang 325035,China)

机构地区：[1]合肥工业大学微电子学院,安徽合肥230009 [2]温州大学电气与电子工程学院,浙江温州325035

出　　处：《传感技术学报》2023年第4期667-672,共6页Chinese Journal of Sensors and Actuators

基　　金：国家重点研发计划项目(2019YFC1605302);国家自然科学基金项目(62074047);安徽省自然科学基金项目(1908085MF180)。

摘　　要：基于适配体修饰的金叉指电极(IDE),研发了一种简单灵敏的生物传感器,用于实现对超低浓度黄曲霉毒素B1(AFB1)的快速检测。在不使用额外预富集装置与步骤的前提下,利用交流电热微流体作用实现对AFB1分子的快速定向驱动与富集,并通过界面电容传感机制反映被测溶液中AFB1的浓度。该传感器集目标物富集和信号检测过程于一体,从加样到结果的时间短至30 s,并具有较宽的线性范围(1 fmol/L~10 pmol/L),其检出限低至0.472 fmol/L。同时,通过选择性实验验证了该传感器对AFB1的特异性识别功能,其对非目标物的选择比为2296∶1。所提出的生物传感器及其检测方法为快速检测食品中的AFB1提供了一种具有竞争力的解决方案。To fast and conveniently detect aflatoxin B1(AFB1),a simple and sensitive biosensor has been developed for real⁃time detection of AFB1 based on an aptamer⁃modified gold interdigital electrode(IDE).Without any additional apparatuses or steps for pre⁃concentration,target enrichment and quantitative detection of AFB1 are realized via microfluidic AC electrothermal effect and interface ca⁃pacitance sensing,respectively.This biosensor combines target enrichment with detection in a single process with a response time as short as 30 s from⁃sample⁃to⁃result.Meanwhile,it achieves a wide linear range(1 fmol/L~10 pmol/L),a detection limit of 0.472 fmol/L and a good selectivity of 2296∶1.the proposed biosensor as well as the detection strategy provides a competitive solution for rapid detection of trace AFB1 in food.

关 键 词：生物传感器 黄曲霉毒素B1检测 交流电热效应 适配体 界面电容 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]