超快速液相色谱-质谱法测定鲜人参晶及林下山参中的39个人参皂苷成分  

Determination of 39 Ginsenosides in Fresh Ginseng Crystals and Understory Ginseng by Ultra-fast Liquid Chromatography-Mass Spectrometry

作　　者：陈丽华 杨秀伟[1] 白雪媛 赵大庆 CHEN Li-hua;YANG Xiu-wei;BAI Xue-yuan;ZHAO Da-qing(State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China;Jilin Ginseng Academy,Changchun University of Traditional Chinese Medicine,Changchun 130000,China)

机构地区：[1]北京大学药学院天然药物学系/天然药物及仿生药物国家重点实验室,北京100191 [2]长春中医药大学吉林省人参科学研究院,吉林长春130000

出　　处：《中国现代中药》2023年第4期788-797,共10页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81973446)。

摘　　要：目的:通过超快速液相色谱-质谱法(UFLC-MS/MS)同时测定鲜人参晶和林下山参中主要活性成分人参皂苷的含量,探讨鲜人参晶及林下山参中人参皂苷的差异变化,为阐明鲜人参晶物质基础提供参考。方法:室温下,用70%甲醇水溶液对鲜人参晶及林下山参药材进行超声提取,采用Waters ACQUITY UPLC■BEH Shield RP C_(18)色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.5 mmol·L^(–1)甲酸铵水溶液为流动相梯度洗脱。在电喷雾离子源负离子、多反应监测模式下,通过UFLC-MS/MS外标对照品法测定鲜人参晶及林下山参中39个人参皂苷的含量。结果:经方法学验证,建立的分析方法符合定量分析要求。4批林下山参和1批鲜人参晶中的总人参皂苷质量分数分别为27.59~52.63、52.26 mg·g^(–1),稀有人参皂苷质量分数分别为3.79~6.65、7.42 mg·g^(–1)。林下山参中人参皂苷Rg1和人参皂苷Re总质量分数为0.72%~0.94%,人参皂苷Rb1质量分数为0.24%~0.53%;鲜人参晶中人参皂苷Rg1和人参皂苷Re总质量分数为1.21%,人参皂苷Rb1质量分数为0.43%。结论:所检测的鲜人参晶和林下山参中人参皂苷Rg1和人参皂苷Re总量及人参皂苷Rb1含量均符合《中华人民共和国药典》2020年版质量标准规定;采用现代工艺制成的鲜人参晶中含有丰富的人参皂苷,尤其是稀有人参皂苷,其与林下山参中的人参皂苷种类几乎无变化,但含量有一定差异。研究结果为综合评价林下山参和鲜人参晶的质量与功效提供了参考。Objective:To determine the contents of ginsenosides in fresh ginseng crystals and understory ginseng by ultra-fast liquid chromatography-mass spectrometry(UFLC-MS/MS),so as to study the difference of ginsenosides in fresh ginseng crystals and understory ginseng,and to provide references for elucidating the material basis of fresh ginseng crystals.Methods:Fresh ginseng crystals and understory ginseng samples were ultrasonic-extracted with 70%methanol aqueous solution at room temperature.UFLC-MS/MS analysis was performed on Waters ACQUITY UPLC■BEH Shield RP C_(18) column(100 mm×2.1 mm,1.7μm),together with a gradient elution using acetonitrile and 0.5 mmol·L^(–1)ammonium formate in water as mobile phases.Under the electrospray ion source,negative ion and multiple reaction monitoring mode,the contents of 39 ginsenoside analytes in fresh ginseng crystals and understory ginseng were determined by the external standard reference method.Results:After methodological verification,the analytical method established in this study met with the requirements of quantitative analysis.The contents of total ginsenosides in 4 batches of understory ginseng and 1 batch of fresh ginseng crystals were 27.59-52.63 and 52.26 mg·g^(–1),respectively,while the total contents of rare ginsenosides were 3.79-6.65 and 7.42 mg·g^(–1),respectively.The total mass fractions of ginsenoside Rg1 and ginsenoside Re in understory ginseng and fresh ginseng crystals were 0.72%-0.94%and 1.21%,while the contents of ginsenoside Rb1 were 0.24%-0.53%and 0.43%,respectively.Conclusion:The total amount of ginsenoside Rg1 and ginsenoside Re as well as the content of ginsenoside Rb1 in fresh ginseng crystals and understory ginseng were in accordance with the specifications in current quality standards in Pharmacopoeia of the People's Republic of China(2020 edition).The fresh ginseng crystals processed by modern technology were rich in ginsenosides,especially rare ginsenosides,which were almost the same as those in understory ginseng,although the content

关 键 词：鲜人参晶 林下山参 人参皂苷 超快速液相色谱-质谱法 

分 类 号：R284[医药卫生—中药学]