芪丹方主成分抑制肝星状细胞增殖及最佳配伍剂量  

Inhibition of Hepatic Stellate Cell Proliferation by the Principle Components of Qidan Prescription and the Optimal Compatible Dose

作　　者：董人华 徐焕焕 王秀丽[1] 李耿 曲舒显 DONG Ren-hua;XU Huan-huan;WANG Xiu-li;LI Geng;QU Shu-xian(Beijing University of Chinese Medicine,Beijing 100102,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China)

机构地区：[1]北京中医药大学,北京100102 [2]中国医学科学院,北京协和医学院,药用植物研究所,北京100193

出　　处：《中国现代中药》2023年第4期859-866,共8页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81202928)。

摘　　要：目的:对芪丹方中的主成分丹酚酸B(Sal B)、丹参酮ⅡA(TSN)、白藜芦醇(Res)、黄芪甲苷(AsA)和甘草次酸(GA)进行配伍研究,以确定芪丹方最佳有效成分之间的配伍比例。方法:采用甲基噻唑基二苯基四唑溴化铵(MTT)法观察不同药物对肝星状细胞(HSC)增殖的抑制作用,单因素法筛选抗肝纤维化的最佳浓度;在单因素试验的基础上,采用响应面法筛选抗肝纤维化的处方。结果:确定最佳配伍为TSN∶Sal B∶AsA=5.57∶5.96∶5.55(摩尔比),对HSC增殖的抑制率可达97.45%;各组分单独给药则显示Sal B、TSN、AsA和GA对HSC增殖的抑制能力较强,Res的抑制能力较弱,且TSN、Sal B和AsA的浓度与抑制率有较大的联系;TSN的抑制效果最好,抑制率为24.19%。结论:Sal B、TSN、Res、AsA和GA单独给药均可抑制HSC的增殖,并呈浓度依赖性。联合用药时,TSN、Sal B和AsA对HSC增殖的抑制作用明显增强。Objective:Qidan Prescription is a prescription for the treatment of liver fibrosis.The compatibility of salvianolic acid B(Sal B),tanshinoneⅡA(TSN),resveratrol(Res),astragalosideⅣ(AsA)and glycyrrhetinic acid(GA)in Qidan Prescription was studied to determine the compatibility of the optimal effective components of Qidan Prescription.Methods:MTT method was used to observe the inhibitory effect of different drugs on the proliferation of hepatic stellate cells(HSCs),and the concentration of antihepatic fibrosis was screened by single factor method.On the basis of single factor experiment,the prescription of antihepatic fibrosis was screened by response surface methodology.Results:The optimal prescription was determined as TSN:Sal B:AsA=5.57:5.96:5.55(mole ratio),with the inhibition rate of HSC proliferation up to 97.45%.The administration of each component alone unveiled potent inhibition of HSC proliferation by Sal B,TSN,AsA and GA and weak inhibition by Res.Moreover,the concentrations of TSN,Sal B and AsA were significantly correlated with the inhibition rates.TSN exhibited the best inhibitory effect with the inhibition rate of 24.19%.Conclusion:Administration of Sal B,TSN,Res,AsA and GA alone all inhibited the proliferation of HSCs in a concentration dependent manner.The inhibitory effect on the proliferation of HSC was significantly increased by combined drug administration,especially by TSN,Sal B and AsA.

关 键 词：肝星状细胞 芪丹方 丹酚酸B 丹参酮ⅡA 甲基噻唑基二苯基四唑溴化铵法 响应面法 

分 类 号：R286[医药卫生—中药学]