Solid-phase microextraction and cuticular hydrocarbon differences related to reproductive activity in juniper bark borer Semanotus bifasciatus Motschulsky  

作　　者：Jia-Xing Fang Da-Feng Chen Xia Shi Su-Fang Zhang Fu Liu Wei-Xing Shen Chun-Yan Jia Shen-Cheng Ma Zhen Zhang Xiang-Bo Kong 

机构地区：[1]Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China [2]Key Laboratory of Silviculture in Downstream Areas of the Yellow River of National Forestry and Grassland Administration,College of Forestry,Shandong Agricultural University,Tai'an 271018,Shandong,China [3]Taishan Forest Pest Control and Quarantine Station,Taishan Scenic Area Management Committee,Tai'an 271000,Shandong,China

出　　处：《Journal of Systematics and Evolution》2023年第3期498-505,共8页植物分类学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31470654);part of the financial support came from the program“Research and Demonstration of Key Technologies for Monitoring,Early Warning and Prevention of Phloeosinus aubei in Taishan Nature Reserve.”。

摘　　要：Cuticular hydrocarbons of Cerambycidae species can function as signals for sex recognition.Little is known about the copulatory signals of the juniper bark borer Semanotus bifasciatus,a major economic threat to Platycladus orientalis Franco in China.Here,we investigated the cuticular hydrocarbons of both sexes of S.bifasciatus to determine the chemically mediated mating signals using the solid-phase microextraction(SPME)technique with carbowax/divinylbenzene fibers(CAR/DVB)and then analyzed by coupled gas chromatography-mass spectrometry(GC-MS).A series of aliphatic saturated straight-chain n-alkanes(n-C_(23) to n-C_(28)),internally branched monomethylalkanes at carbons 3,11,or 13,and dimethylalkanes were identified,which showed no qualitative differences in either sex and were similar in the samples with SPME fiber extraction and those with hexane extraction.The bioassay showed that 11-methylpentacosane(11-MeC_(25)),11-methylhexacosane(11-MeC_(26)),and 11-methylheptacosane(11-MeC_(27))have sex-specific recognition functions that triggered more mating attempts at a female-specific ratio of 100:4:60 than at a male-specific ratio of 100:85:50.In addition,the female-specific ratio of 11-methylalkanes can elicit about 70%of male mating attempts within about 60 s,whereas live females elicit about 98%of male mating attempts within 25 s.The discrepancy in the initiation of mating attempts by synthetic mixtures and live females suggests that the methyl isomers 3-MeC_(25),3-MeC_(27),and/or 11,15-diMeC_(27) may also be involved in the mating behavior of S.bifasciatus.These results suggest that 11-MeC_(25),11-MeC_(26),and 11-MeC_(27) constitute the contact sex pheromone of S.bifasciatus,with the presence or absence of 11-MeC_(26) in particular playing an important role in mate recognition by males.

关 键 词：cuticular hydrocarbons GC-MS mating behavior SEMIOCHEMICALS sex recognition 

分 类 号：O62[理学—有机化学]