玉米NF-YB家族基因的鉴定与功能研究  

Identification and Functional Study of NF-YB Family Genes in Maize(Zea mays)

作　　者：刘建涵 马宇馨 孙雨彤 樊梓萱 邢继红[1,2] 张康 LIU Jian-Han;MA Yu-Xin;SUN Yu-Tong;FAN Zi-Xuan;XING Ji-Hong;ZHANG Kang(State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071000,China;Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学华北作物改良与调控国家重点实验室,保定071000 [2]河北农业大学河北省植物生理与分子病理学重点实验室,保定071000

出　　处：《农业生物技术学报》2023年第6期1111-1121,共11页Journal of Agricultural Biotechnology

基　　金：国家玉米产业技术体系(CARS-02);河北省高等学校科学研究项目(BJK2022006);河北省省属高等学校基本科研业务费研究项目(KY2021043,KY2021044);大学生创新创业训练项目(202110086004)。

摘　　要：核转录因子Y(nuclear factor Y,NF-Y)由NF-YA、NF-YB、NF-YC 3个亚单位组成,能够特异性识别CCAAT-BOX,是真菌、动物和植物等众多真核生物基因中存在最广泛的启动子元件之一。为了鉴定玉米(Zea mays)NF-YB家族成员并分析其在不同组织和生理条件下的表达规律,本研究利用生物信息学方法对玉米基因组中NF-YB编码基因进行了系统性鉴定和分析,并利用茉莉酸、水杨酸和乙烯喷施自交系B73植株,测定该家族成员在激素处理下的表达规律。结果表明,玉米NF-YB基因家族成员共18个,均有NFYB结构域,上游具有水杨酸、茉莉酸和脱落酸结合位点。玉米NF-YB家族基因在水杨酸、茉莉酸和脱落酸处理后,表达水平在24 h之内就有明显的升高,预测NF-YB参与水杨酸、茉莉酸和脱落酸途径。在冷、热、紫外辐射胁迫下,家族基因整体表达水平下调;在盐和干旱胁迫下,家族基因整体表达水平升高。在禾谷镰刀菌(Fusarium graminearum)侵染下,Zm00001d042196、Zm00001d010574、Zm00001d019101表达水平升高。本研究对玉米NF-YB家族基因进行了系统性鉴定和分析,明确了玉米NF-YB家族成员,揭示了玉米NF-YB家族成员在玉米生物和非生物胁迫中的表达规律,为进一步阐明其功能提供了重要的理论基础。Nuclear transcription factor Y(NF-Y)is composed of 3 subunits,NF-YA,NF-YB,and NF-YC,and can specifically recognize CCAAT-BOX.In order to identify the members of NF-YB family in maize(Zea mays)and analyze their expression patterns in different tissues and physiological conditions,the NF-YB coding genes in maize genome were systematically identified and analyzed by bioinformatics methods.The expression patterns of NF-YB family members under hormone treatment were determined by spraying inbred line B73 plants with salicylic acid,jasmonic acid,and abscisic acid.The results showed that there were 18 members of the maize NF-YB gene family,all of which had NFYB domains and upstream binding sites for salicylic acid,jasmonic acid and abscisic acid.The experiment proved that the expression levels of maize NF-YB family genes increased significantly within 24 h after salicylic acid,jasmonic acid and abscisic acid treatment.It was predicted that NF-YB was involved in salicylic acid,jasmonic acid and abscisic acid pathway.Under cold,heat and UV stresses,the overall expression levels of family genes were down regulated;under salt and drought stress,the overall expression levels of family genes were increased.Under the infection of Fusarium graminearum,the expression levels of Zm00001d042196,Zm00001d010574 and Zm00001d019101 were increased.In this study,the maize NF-YB family genes were systematically identified and analyzed,the members of the maize NF-YB family were clarified,and the expression rules of maize NF-YB family members in maize biotic and abiotic stresses were revealed.This study provides an important theoretical basis for further elucidating the function of this family.

关 键 词：玉米 核转录因子Y(NF-YB) 转录因子 表达 胁迫 

分 类 号：S432.1[农业科学—植物病理学]