基于农艺性状和InDel标记的矮生番茄种质资源遗传多样性分析  

Genetic Diversity Analysis of Dwarf Tomato Germplasm Resources Based on Agronomic Traits and InDel Markers

作　　者：阚云霞 师海林 张丹丹 游茜 尤园园 舒金帅[4] 王帅[1,2,3] 毛秀杰 KAN Yun-Xia;SHI Hai-Lin;ZHANG Dan-Dan;YOU Xi;YOU Yuan-Yuan;SHU Jin-Shuai;WANG Shuai;MAO Xiu-Jie(College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Hebei Key Laboratory of Characteristic Horticultural Germplasm Mining and Innovative Utilization,Qinhuangdao 066004,China;Hebei Higher Institute Application Technology Research and Development Center of Horticultural Plant Biological Breeding,Qinhuangdao 066004,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]河北科技师范学院园艺科技学院,秦皇岛066004 [2]河北省特色园艺种质挖掘与创新利用重点实验室,秦皇岛066004 [3]河北省高校特色园艺植物生物育种应用技术研发中心,秦皇岛066004 [4]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《农业生物技术学报》2023年第6期1122-1132,共11页Journal of Agricultural Biotechnology

基　　金：河北省重点研发计划(20326325D);河北省自然科学基金(C2022407068);河北省省属高校基本科研业务费专项(2021JK07);河北省燕山农业特色产业技术研究院绩效后补助经费(205676154H)。

摘　　要：矮生番茄(Solanum lycopersicum)兼具观赏和食用价值,丰富的种质资源是矮生番茄种质资源创新和新品种选育的基础。本研究对45份国内外矮生番茄种质资源的25个性状进行调查,并根据农艺性状进行遗传多样性分析以及聚类分析;利用33对In Del引物对45份矮生番茄种质资源进行PCR扩增,分析其遗传多样性。结果表明,45份矮生番茄种质资源的25个性状的遗传多样性指数范围为0.26~2.03,7个质量性状多样性指数变化范围为0.26~1.31,均值为0.92,18个数量性状变异系数分布在8.51%~32.51%,其均值为19.01%。33对InDel标记检测有效等位基因数均值为1.64,多态性信息含量(polymorphism information content,PIC)范围在0.18~0.43之间,均值为0.31。农艺性状聚类分析与InDel标记聚类分析均将45份材料分为3类,且大部分种质的农艺性状与InDel标记的聚类结果一致性较高。本研究表明,45份矮生番茄种质资源具有丰富的遗传多样性,可为矮生番茄新品种选育提供参考依据。Dwarf tomato(Solanum lycopersicum)has both ornamental and edible values,and its abundant germplasm resources are the basis for germplasm innovating and varieties breeding.In order to study the genetic diversity of germplasm resources and screen more diverse types of germplasm resource materials for parental selection and variety breeding,25 traits of 45 dwarf tomato germplasm resources from domestic and foreign were investigated,and genetic diversity analysis as well as cluster analysis were carried out;33 pairs of InDel primers were used to PCR amplification to analyze their genetic diversity.The results showed that the genetic diversity index of 25 traits ranged from 0.26 to 2.03,the diversity index of the 7 mass traits ranged from 0.26 to 1.31,with the average value of 0.92,and the coefficients of variation of the 18 quantitative traits were distributed from 8.51%to 32.51%,with the average value of 19.01%.The mean number of alleles effective for InDel marker detection was 1.64,and the polymorphic information content(PIC)ranged from 0.18 to 0.43,with the average value of 0.31.Both agronomic trait cluster analysis and InDel marker cluster analysis divided 45 materials into 3 categories,and the agronomic traits of most germplasm were consistent with the clustering results of InDel markers.The results showed that 45 dwarf tomato germplasm resources had rich genetic diversity,which could provide reference basis for new varieties breeding.

关 键 词：矮生番茄 种质资源 农艺性状 In Del标记 遗传多样性 

分 类 号：S641.2[农业科学—蔬菜学]