基于转录组数据分析不同精粗比例日粮对绵羊腿脂和大网膜脂中脂质代谢的影响  

Analysis of Effects of Different Concentrate/Forage Ratio Diets on Lipid Metabolism in Femoral and Omental Adipose Tissue of Sheep(Ovis aries)Based on Transcriptome Data

作　　者：杨金丽[1] 吴孟楠 高智雄 王雅宁 李瑞雪 邵雨萌 王海荣[1] 高爱武[1] YANG Jin-Li;WU Meng-Nan;GAO Zhi-Xiong;WANG Ya-Ning;LI Rui-Xue;SHAO Yu-Meng;WANG Hai-Rong;GAO Ai-Wu(Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学动物科学学院/内蒙古自治区高校动物营养与饲料科学重点实验室,呼和浩特010018

出　　处：《农业生物技术学报》2023年第6期1206-1217,共12页Journal of Agricultural Biotechnology

基　　金：内蒙古自治区高校科研重点项目(NJZZ23040);内蒙古农业大学动科院青年基金(QN202118,QN201916);内蒙古农业大学标志性成果专项基金(BZCG202011)。

摘　　要：饲喂高精料日粮常引起反刍动物内脏脂肪过度沉积和代谢病高发。本研究应用高通量测序技术对饲喂不同精粗比例日粮绵羊腿脂和大网脂的脂质代谢进行系统分析。研究选用(90±15)d健康杜蒙公羔羊(Ovis aries)15只,随机分为3组,分别饲喂精料比例为30%(G30组)、50%(G50组)和70%(G70组)的日粮,预饲期15 d,正式饲养60 d后进行屠宰,每组取接近平均体重的3只羊的腿脂和大网膜脂进行转录组测序。对绵羊腿脂和大网脂中不同日粮组间表达量差异大于10%以上的基因(differentially expressed gene,DEG)进行KEGG通路富集分析,同时对脂质代谢关键酶基因表达量变化进行分析并应用qPCR进行验证。结果发现,G50组绵羊干物质采食量显著(P<0.05)高于G70组和G30组,而G50组和G70组绵羊60 d体重显著(P<0.05)高于G30组。G50组绵羊与G30组相比,脂质代谢通路中脂肪酸合成、延伸、去饱和、甘油三酯代谢和脂肪酸降解在腿脂和大网脂组织中均升高。G70组绵羊与G30组相比,脂肪酸延伸、去饱和与脂肪酸降解在腿脂和大网脂组织均升高。G50组绵羊与G70组相比,脂肪酸合成与去饱和在腿脂和大网脂组织均升高。不同精料比例日粮对绵羊腿脂和大网脂影响差异表现为:G70组绵羊与G30组相比仅大网脂中DEG在甘油三酯代谢通路显著(P_(adjust)<0.05)富集。G50组绵羊与G70组相比仅腿脂中DEG在脂肪酸延伸与甘油三酯代谢显著(P_(adjust)<0.05)富集,而2组间仅大网脂DEG在脂肪酸降解通路显著(P_(adjust)<0.05)富集。q PCR结果显示,G50组绵羊腿脂中脂肪酸合成酶和硬脂酸去饱和酶基因表达量显著(P<0.05)高于G30和G70组,与转录组测序结果一致。本研究揭示了绵羊腿脂和大网脂在不同精粗比例日粮下脂质代谢变化的异同,为降低内脏脂肪沉积,提高动物养殖效率提供理论依据。Feeding high-concentrate diets often causes excessive visceral fat deposition and high incidence of metabolic diseases in ruminants.In this study,high throughput sequencing technology was used to systematically analyze the lipid metabolism of femoral and omental adipose tissue in sheep fed with different concentrate to forage ratios diets.15 healthy Dumont male lambs(Ovis aries)aged(90±15)days were selected and randomly divided into 3 groups.Diets concentrate ratio were 30%(G30),50%(G50),and 70%(G70).After 15 d of adaptation,sheep were raised for 60 d and slaughtered at the end.Femoral and omental fat from 3 lamb that close to average body weight of every group were sampled for transcriptome sequencing.KEGG pathway enrichment analysis were conducted to study more than 10%differentially expressed gene(DEG)between different groups.Relative gene expression of lipid metabolism related key enzymes in femoral fat were also determined by qPCR.The results showed that the dry matter intake of sheep in G50 group were significantly(P<0.05)higher than those in G70 and G30 groups.The body weight of sheep in G50 and G70 groups after 60 days of feeding was significantly(P<0.05)higher than that in G30 group.KEGG pathway enrichment result and essential gene expression changes combined to indicate fatty acid synthesis,elongation,desaturation,triglyceride metabolism and fatty acid degradation in femoral and omental fat of G50 group were all higher than those in the G30 group.The fatty acid elongation,desaturation and fatty acid degradation of femoral and omental fat in G70 group were higher than those in G30 group.The fatty acid synthesis and desaturation of femoral and omental fat in G50 group were higher than those in G70 group.The differential impact of diets concentrate ratio on femoral or omental fat were as follow:The triglyceride metabolism of omental fat in G70 group was higher than that in G30 group(P_(adjust)<0.05).The fatty acid elongation and triglyceride metabolism of femoral fat in G50 group were higher(P_(adjust)<0.

关 键 词：绵羊 转录组 精料比例 脂肪组织 脂质代谢 

分 类 号：S826[农业科学—畜牧学]