普鲁士蓝处理AML细胞WEHI-3制备的疫苗抗皮下瘤效应  

Antagonistic Effect of Vaccine Prepared from PBNPS⁃Treated WEHI⁃3 Cells on Acute Myeloid Leukemia⁃Growth in Mice

作　　者：包雪阳 徐慧 赵枫姝[1] 张宇[2] 窦骏[1] BAO Xue-yang;XU Hui;ZHAO Feng-shu;ZHANG Yu;DOU Jun(Department of Pathogenic Biology and Immu-nology,School of Medicine,Southeast University,Nanjing210000,China;School of Biological Sciences and Medical Engineering,Southeast University,Nanjing210000,China)

机构地区：[1]东南大学医学院病原生物学与免疫学系,南京210000 [2]东南大学生物科学与医学工程学院,南京210000

出　　处：《中国药学杂志》2023年第9期786-791,共6页Chinese Pharmaceutical Journal

基　　金：国家重点研发计划项目资助(2017YFA0205502)。

摘　　要：目的 探究经普鲁士蓝纳米颗粒(prussianblue nanoparticles, PBNPs)处理的小鼠急性髓系白血病(acute myeloid leukemia, AML)细胞系WEHI-3所制备的疫苗,对AML皮下瘤生长的拮抗作用,为PBNPs用于AML免疫治疗研究提供新策略。方法 用无血清培养基(serum free medium, SFM)和PBNPs处理WEHI-3细胞,观察细胞形态的改变,并检测干性相关基因的表达;用PBNPs处理过的1×10^(6)个WEHI-3细胞反复冻融法制备AML细胞疫苗,免疫BALB/c小鼠3次10 d后,再用1×10^(5)个WEHI-3细胞攻击免疫鼠,观察不同处理疫苗免疫鼠皮下瘤生长状况。结果 SFM处理WEHI-3细胞14 d后和PBNPs处理WEHI-3细胞3 d后,细胞悬浮成团,干性相关基因的表达上调;PBNPs处理的WEHI-3细胞疫苗免疫鼠,与其他对照组相比,生存期更长、皮下瘤块更小。结论 PBNPs处理的WEHI-3细胞系可以诱导其发生干细胞样变化,用其制备的细胞疫苗免疫鼠,具有拮抗AML皮下瘤生长效应。OBJECTIVE To investigate anti⁃tumor effect of vaccine prepared from WEHI⁃3 cells treated with prussianblue nanop⁃articles(PBNPs)and provide new strategy for the study of PBNPs in acute myeloid leukemia(AML)immunotherapy.METHODS WEHI⁃3 cells were treated with serum free medium(SFM)and PBNPs,then the morphological changes were observed and the expression of stemness⁃related genes were detected.Meanwhile,tumor vaccine,prepared by repeated freezing and thawing of 1×10^(6)PBNPs⁃treated WEHI⁃3 cells,was used to immunize BALB/c mice for three times.Ten days after the last immunization,the mice were hypodermically injected with 1×10^(5)WEHI⁃3 cells and its tumor size were recorded.RESULTS WEHI⁃3 cells formed cel⁃lular suspension clusters after being treated with SFM for 14 days and PBNPs for 3 days,and stemness⁃related genes were up⁃regulated both in mRNA and protein levels.The mice immunized by PBNPs⁃treated vaccine had longer survival and smaller tumor size compared with the controls.CONCLUSION PBNPs can induce stem⁃like characteristic changes in WEHI⁃3 cell line.Vaccine prepared from PBNPs⁃treated WEHI⁃3 cells has antagonistic effect on tumor growth.

关 键 词：急性髓系白血病 普鲁士蓝纳米颗粒 WEHI-3细胞系 疫苗 抗急性髓系白血病效应 

分 类 号：R967[医药卫生—药理学] R733.3[医药卫生—药学] R392.5