水禽源大肠杆菌耐药基因和质粒的相关性研究  

Correlation study on drug resistance genes and plasmids in waterfowl-derived Escherichia coli

作　　者：冯曼玉 缪娟娟 杨跃飞[1,2] 王彦红 FENG Manyu;MIAO Juanjuan;YANG Yuefei;WANG Yanhong(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Provincial Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《黑龙江畜牧兽医》2023年第9期80-86,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：江苏高校优势学科建设工程项目(PAPD);扬州大学大学生创新创业训练计划基金项目(x20200702)。

摘　　要：为了探究水禽源大肠杆菌的耐药性、耐药基因携带情况及其与质粒的相关性,试验用纸片扩散法检测62株水禽源大肠杆菌对14种抗菌药物的耐药性,用PCR方法对13种耐药基因和18种质粒进行扩增,并对质粒检出率较高的阳性菌株进行耐药基因的检测。结果表明:62株水禽源大肠杆菌对阿莫西林、美洛西林和链霉素的耐药性较高,耐药率分别为88.7%、74.2%和51.6%;对丁胺卡那和头孢哌酮耐药性较低,耐药率分别为3.2%和1.6%。aac(6′)-Ib、TEM、qnrD及qnrS基因的检出率较高,分别为66.1%、59.7%、46.8%和29.0%;共检出6种质粒IncFIA、IncFIB、IncY、IncI1、IncHI2、IncN,其中质粒IncFIB的检出率最高,为80.6%。IncFIB阳性菌株的aac(6′)-Ib基因检出率为72.0%,qnrS基因检出率为22.0%。说明水禽源大肠杆菌耐药性高,常见耐药基因以aac(6′)-Ib为主,常见质粒以IncFIB为主,质粒IncFIB介导的aac(6′)-Ib基因传播较为流行。In order to explore the drug resistance and the carriage of drug resistance genes of waterfowl-derived Escherichia coli, and their correlation with plasmids, in this experiment, the K-B method was used to detect the resistance rates of 14 kinds of antimicrobial drugs among 62 strains of waterfowl-derived Escherichia coli. The 13 kinds of drug resistance genes and 18 kinds of plasmids were amplified by PCR, and the drug resistance genes were detected in the plasmid-positive strains with high detection rate. The results showed that 62 strains of waterfowl-derived E. coli had high drug resistance to amoxicillin, mezlocillin and streptomycin, with drug resistance rates of 88.7%, 74.2% and 51.6%, respectively, and had low drug resistance to amikacin and cefoperazone, with drug resistance rates of 3.2% and 1.6%, respectively. The detection rates of aac(6′)-Ib, TEM, qnrD and qnrS genotypes were high, being 66.1%, 59.7%, 46.8% and 29%, respectively. Six plasmids including IncFIA, IncFIB, IncY, IncI1, IncHI2, IncN were detected, among which the detection rate of plasmid IncFIB was the highest, being 80.6%. The detection rate of the aac(6′)-Ib gene was 72.0% and the qnrS gene detection rate was 22.0% for the IncFIB-positive strain. The results suggested that the waterfowl-derived E. coli had high drug resistance. The common drug resistance gene was mainly aac(6′)-Ib, and the common plasmid was mainly IncFIB. Plasmid IncFIB-mediated transmission of the aac(6′)-Ib gene was prevalent.

关 键 词：水禽 大肠杆菌 药敏试验 耐药性 耐药基因 质粒 

分 类 号：S839[农业科学—畜牧学]