野生大型真菌总黄酮含量比较分析  

Comparative Analysis of Total Flavonoids Content in Wild Macrofungi

作　　者：巫桂芬 严焕丹 WU Guifen;YAN Huandan(Guangxi Minzu Normal University,Chongzuo,Guangxi 532200)

机构地区：[1]广西民族师范学院,广西崇左532200

出　　处：《中国农学通报》2023年第13期125-129,共5页Chinese Agricultural Science Bulletin

基　　金：全国大学生创新创业项目“基于互联网模式下黄金菇的营销策略初探”(202110604039X);广西自然科学基金项目“中国-东盟边境地区珍稀野生桑黄资源挖掘及其亲缘关系分析”(2022GXNSFBA035524);广西壮族自治区教育厅第四批民族院校特色学科建设立项建设学科项目(民族生态学);广西民族师范学院人才启动项目“桑树桑黄在桂西南地区的引种与栽培技术试验示范”(2020BS002)。

摘　　要：为探究左江流域大型真菌资源总黄酮含量分布规律,以木蹄层孔菌、迷宫栓孔菌、瓦尼纤孔菌、变形薄孔菌、药用拟层孔菌、忍冬纤孔菌、膨大革孔菌、桦附毛孔菌8种大型真菌为实验材料,采用超声波法从真菌中提取总黄酮,并用亚硝酸钠-硝酸铝显色法测定其总黄酮含量。在510 nm处,芦丁标准品浓度与吸光值的线性关系良好,线性相关系数R2=0.9861。8种大型真菌均含有黄酮类化合物,其中瓦尼纤孔菌含量最高,为39.740 mg/g;桦附毛孔菌含量最低,为5.532 mg/g。The paper aims to explore the distribution of total flavonoids in macrofungi resources in the Zuojiang River Basin.Eight kinds of macrofungi were used as experimental materials,including Pyropolyporus fomentarius,Trametes gibbosa,Phellinus vaninii,Antrodia variifoormis,Fomitopsis officinalis,Inonotus lonicericola,Coriolopsis strumsa,and Trichaptum pargamenum.Total flavonoids were extracted from the above macrofungi by ultrasonic method,and the contents of total flavonoids were determined by sodium nitrite method.The results showed that the linear relationship between the concentration of rutin standard and the absorbance value was good at 510 nm,and the linear correlation coefficient was R^(2)=0.9861.The eight macrofungi all contained flavonoids,and Phellinus vaninii had the highest content of flavonoids of 39.740 mg/g,while Trichaptum pargamenum had the lowest content of 5.532 mg/g.

关 键 词：大型真菌 野生 超声波 黄酮 

分 类 号：S646[农业科学—蔬菜学]