苗药五香血藤醇提物对慢性非细菌性前列腺炎大鼠模型前列腺组织白细胞计数及卵磷脂小体密度的影响  

Effect of alcohol extract of Miao Medicine spiced blood cane on white blood cell counts and lecithin corpuscles density in prostate tissues of rat model with chronic abacterial prostatitis

作　　者：李章春[1] 孙海波[2] 杨政敏[3] 党荣敏[1] 李珀 LI Zhangchun;SUN Haibo;YANG Zhengmin;DANG Rongmin;LI Po(Section of Basic Medicine,Qiannan Medical College for Nationality,Duyun,558000,Guizhou,China;Section of Public Course Teaching,Qiannan Medical College for Nationality,Duyun,558000,Guizhou,China;School of Pharmacy,Qiannan Medical College for Nationality,Duyun,558000,Guizhou,China;Department of Pathology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]黔南民族医学高等专科学校基础医学部,贵州省都匀市558000 [2]黔南民族医学高等专科学校公共课教学部,贵州省都匀市558000 [3]黔南民族医学高等专科学校药学系,贵州省都匀市558000 [4]贵州医科大学附属医院病理科,贵州省贵阳市550004

出　　处：《广西医学》2023年第7期792-795,810,共5页Guangxi Medical Journal

基　　金：贵州省卫生健康委科学技术基金项目(gzwkj2021-384);黔南民族医学高等专科学校科研基金项目(qnyz202033)。

摘　　要：目的探讨苗药五香血藤醇提物对慢性非细菌性前列腺炎(CAP)大鼠模型前列腺组织白细胞计数及卵磷脂小体密度的影响。方法将60只雄性健康SD大鼠分为正常对照组、CAP模型对照组(模型组)、阳性药物对照组(阳性药物组)、五香血藤醇提物低剂量组(低剂量组)、五香血藤醇提物中剂量组(中剂量组)、五香血藤醇提物高剂量组(高剂量组),每组10只。除正常对照组大鼠外,其余各组大鼠采用25%消痔灵制备CAP大鼠模型。建模7 d后,阳性药物组灌胃20 mL/kg前列舒通胶囊水溶液,低剂量组、中剂量组、高剂量组分别灌胃63 mg/kg、126 mg/kg、252 mg/kg五香血藤醇提物,模型组和正常对照组灌胃20 mL/kg无菌生理盐水,连续灌胃28 d。末次灌胃后2 h,处死各组大鼠并分离前列腺组织,观察并计算各组大鼠前列腺组织的白细胞计数及卵磷脂小体密度,采用HE染色法观察各组大鼠前列腺组织的病理学改变。结果与正常对照组相比,模型组大鼠的前列腺组织白细胞计数升高,卵磷脂小体密度降低(均P<0.05);与模型组相比,阳性药物组、低剂量组、中剂量组、高剂量组大鼠的前列腺组织白细胞计数均降低,卵磷脂小体密度均升高,且阳性药物组、高剂量组大鼠的前列腺组织白细胞计数均低于低剂量组与中剂量组,卵磷脂小体密度均高于低剂量组与中剂量组(均P<0.05)。HE染色结果显示,模型组大鼠的前列腺组织内有明显的慢性炎症改变,有大量淋巴细胞等炎症细胞浸润,腺体上皮细胞及纤维组织增生;与模型组相比,阳性药物组、低剂量组、中剂量组、高剂量组大鼠的前列腺组织慢性炎症病变均有不同程度改善,以高剂量组及阳性药物组的改善程度更为明显。结论五香血藤醇提物能够降低CAP大鼠模型前列腺组织的白细胞计数,升高卵磷脂小体密度,改善前列腺组织的炎症病变,以252 mg/kg五香血藤醇提物的干�Objective To explore the effect of alcohol extract of Miao Medicine spiced blood cane on white blood cell counts and lecithin corpuscles density in prostate tissues of rat model with chronic abacterial prostatitis(CAP).Methods A total of 60 male healthy SD rats were assigned to normal control group,CAP model control group(model group),positive drug control group(positive drug group),alcohol extract of spiced blood cane with low dose group(low-dose group),alcohol extract of spiced blood cane with medium dose group(medium-dose group),or alcohol extract of spiced blood cane with high dose group(high-dose group),with 10 rats in each group.Except for rats in the normal control group,rats in the remaining groups received 25%Xiaozhiling for CAP rat model preparation.After 7 days of model establishment,the positive drug group received intragastric administration of 20 mL/kg Qianlie Shutong Capsule aqueous solution,the low-,medium-,and high-dose groups received intragastric administration of 63 mg/kg,126 mg/kg,and 252 mg/kg alcohol extract of spiced blood cane,respectively,and the model group and the normal control group received intragastric administration of 20 mL/kg aseptic normal saline,for a continuous 28-day intragastric administration.After 2 hours of the last intragastric administration,rats in various groups were killed,and their prostate tissues were isolated.The white blood cell counts and lecithin corpuscles density in prostate tissues of rats in various groups were observed and calculated.The pathological changes of prostate tissues in rats of various groups were observed by using HE staining.Results Compared with the normal control group,rats in the model group obtained elevated white blood cell counts,and a decreased lecithin corpuscles density in prostate tissues(all P<0.05).Compared with the model group,the white blood cell counts in prostate tissues were decreased,and lecithin corpuscles density in prostate tissues was elevated in the positive drug group,and the low-,medium-,and high-dose groups,and the

关 键 词：慢性非细菌性前列腺炎 五香血藤 前列腺组织 白细胞计数 卵磷脂小体 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]