一株钠长石分解菌的筛选及其生长特性  

Screening and growth characteristics of an albite-decomposing strain

作　　者：刘雪 温全宝 张雷[1,2] 杨志超 郭建英[1,2] 刘生玉 LIU Xue;WEN Quanbao;ZHANG Lei;YANG Zhichao;GUO Jianying;LIU Shengyu(College of Mining Engineering,Taiyuan University of Technology,Taiyuan 030024,Shanxi,China;Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering,Taiyuan 030032,Shanxi,China;Key Laboratory of In-situ Property-improving Mining of Ministry of Education,Taiyuan University of Technology,Taiyuan 030024,Shanxi,China)

机构地区：[1]太原理工大学矿业工程学院,山西太原030024 [2]山西浙大新材料与化工研究院,山西太原030032 [3]太原理工大学原位改性采矿教育部重点实验室,山西太原030024

出　　处：《微生物学通报》2023年第5期1801-1814,共14页Microbiology China

基　　金：国家自然科学基金(22178245);山西浙大新材料与化工研究院研发项目(2022SX-TD009);山西省基础研究计划(202203021211165)。

摘　　要：【背景】钠长石中钠元素的存在给矿物加工利用带来严重的问题。【目的】获得一株对钠长石具有显著分解效果的菌株,以脱除钠长石中钠元素。【方法】以钠长石为唯一钠源,从内蒙古盐湖土壤中筛选分解能力强且性能稳定的菌株,通过形态学观察、生理生化特性以及16S rRNA基因序列分析对菌株进行鉴定,构建其系统发育树,绘制生长曲线并探究培养条件对菌株生长的影响。【结果】筛选到一株钠长石分解菌,经鉴定为约翰逊不动杆菌(Acinetobacter johnsonii),3−11 h为对数生长期,最佳培养条件:初始pH 9.0,培养温度25℃,盐浓度2%,摇床转速150 r/min,接菌量5%,装液量100 mL/250 mL、牛肉膏为唯一碳源,蛋白胨为唯一氮源。【结论】首次报道约翰逊不动杆菌具有高效分解钠长石的性能,为生物脱钠提供菌株来源。[Background]The sodium in albite poses a challenge to mineral processing and utilization.[Objective]To screen an efficient albite-decomposing strain and to remove the sodium in albite.[Methods]With albite as the sole sodium source,a stable strain with strong decomposition ability was screened from the soil of salt lake in Inner Mongolia.Then,it was identified based on morphological observation,physiological and biochemical characteristics,and 16S rRNA gene sequencing.The phylogenetic tree was constructed and the growth curve was plotted.Moreover,the influence of culture conditions of the growth of the strain was investigated.[Results]An efficient albite-decomposing strain was screened out and identified as Acinetobacter johnsonii,with the logarithmic phase of the 3rd−11th hours.The optimal culture conditions are as follows:initial pH 9.0,temperature of 25℃,2%salt,shaker speed at 150 r/min,inoculum of 5%,liquid volume of 100 mL/250 mL,beef extract as the sole carbon source,and peptone as the sole nitrogen source.[Conclusion]It is reported for the first time that A.johnsonii has the ability to decompose albite,which provides a strain for biological removal of sodium.

关 键 词：钠长石 筛选 约翰逊不动杆菌 生长特性 生物脱钠 

分 类 号：Q939.97[生物学—微生物学]