我国施万菌分布及其分子特征分析  

Distribution and molecular characterization of Shewanella in China

作　　者：黄振洲 于可艺 汪永禄[2] 李颖[3] 高鹤[1] 白雪梅[1] 肖悦 赖玖连 李坤 王多春[1] Huang Zhenzhou;Yu Keyi;Wang Yonglu;Li Ying;Gao He;Bai Xuemei;Xiao Yue;Lai Jiulian;Li Kun;Wang Duochun(National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Ma'anshan Prefectural Center for Disease Control and Prevention,Ma'anshan 243000,Anhui,China;Shunyi District Center for Disease Control and Prevention,Beijing 101300,China;School of Public Health,Lanzhou University,Lanzhou 730000,Gansu,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206 [2]马鞍山市疾病预防控制中心,安徽马鞍山243000 [3]北京市顺义区疾病预防控制中心,北京101300 [4]兰州大学公共卫生学院,甘肃兰州730000

出　　处：《疾病监测》2023年第4期384-390,共7页Disease Surveillance

基　　金：国家科技基础资源调查专项(No.2021FY100904)。

摘　　要：目的探索中国施万菌的种群分布、分子流行病学特征及遗传进化关系,为施万菌的流行病学监测和进化研究提供依据。方法利用基质辅助激光解吸电离-飞行时间质谱(MALDI-TOFMS)联合gyrB基因测序进行施万菌种水平鉴定;利用7个管家基因(16SrRNA、gyrA、gyrB、infB、recN、rpoA和topA)进行多位点序列分型(MLST),利用BioNumerics7.1软件对各个序列类型(ST)构建最小生成树。结果本研究共收集全国201株施万菌,鉴定到10个不同的菌种,其中海藻施万菌所占数量最多。不同分离来源中的施万菌种分布有所差异,临床和食品来源的施万菌种分布相似。MLST将施万菌实验室分离株划分成136个ST型和15个克隆复合体(CCs),其中CC12为海藻施万菌优势克隆群。结论我国分布的施万菌病原谱丰富多样,提示这些菌株具有高度的遗传多样性。Objective To explore the distribution and molecular epidemiological characteristics genetic evolution of Shewanella in China and provide evidence for epidemiological surveillance and evolution research of Shewanella.Methods The identification of Shewanella species was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)combined with gyrB gene sequencing.Multilocus sequence typing(MLST)was performed using seven housekeeping genes(16S rRNA,gyrA,gyrB,infB,recN,rpoA and topA).A minimal spanning tree was constructed for each sequence type(ST)by using software BioNumerics 7.1.Results The 201 Shewanella isolates collected in China were identified as io different species,with S.algae accounting for the largest proportion.There were differences in the distribution of Shewanella among the isolated from different sources,the distribution of S.algae from clinical and food sources was similar.MLST can classified the Shewanella isolates into 136 STs,clonal complex 12(CC12)was the dominant clonal complexes of S.algae.Conclusion The rich pathogenic spectrum of Shewanella distributed in China suggests that the genetic diversity of these strains is high.

关 键 词：施万菌 种群分布 分子分型 遗传多样性 

分 类 号：R211[医药卫生—中医学] R378