黄芩中黄酮化合物提取检测研究进展  

Extraction and Detection of Flavonoid Compounds in Scutellaria baicalensis:A Review

作　　者：李轲 封林林 薛婉莹 唐钰玉 郝敬虹[1] 杨柳[1] LI Ke;FENG Linlin;XUE Wanying;TANG Yuyu;HAO Jinghong;YANG Liu(Beijing Key Laboratory of New Technology in Agricultural Application/National Demonstration Center for Experimental Plant Production Education/Beijing University of Agriculture,Beijing 102206;Beijing Kaixin Green Agricultural Technology Co.,Ltd.,Beijing 100081)

机构地区：[1]农业应用新技术北京市重点实验室/植物生产国家级实验教学示范中心/北京农学院,北京102206 [2]北京开心格林农业科技有限公司,北京100081

出　　处：《中国农学通报》2023年第15期158-164,共7页Chinese Agricultural Science Bulletin

基　　金：北京市种质创制和品种选育联合攻关项目(G20220628003)。

摘　　要：黄芩是中国的传统中药材,具有较高的药用价值。为了从黄芩中提取检测黄酮类物质,归纳总结了黄芩根部及地上部黄酮化合物的提取方法,主要有有机溶剂萃取法、水提取法等;分析了黄芩根部及地上部黄酮化合物的分离纯化方法,主要有柱层析色谱法、高速逆流层析法等;梳理了黄芩根部及地上部黄酮化合物的成分测定方法,有高效液相色谱法、超高效液相色谱法等,并针对黄芩在实际应用中存在的问题提出改善建议,如将实验室研究与工业化生产紧密结合,完善实验设备和提取工艺,更好地为工业生产提供便利;充分利用黄芩资源,创造社会效益等。本文在分析黄酮化合物的基础上,综述了黄芩根部及地上部的黄酮化合物的提取、分离及检测方法的研究进展,为黄芩中黄酮的大规模开发与应用提供技术储备,为进一步研究和推广应用黄芩资源奠定技术基础。Scutellaria baicalensis is a traditional Chinese herbal medicine with high medicinal value.In order to extract and detect flavonoids from Scutellaria baicalensis,the methods of extracting flavonoid compounds from Scutellaria baicalensis roots and aboveground parts are summarized,such as organic solvent extraction,water extraction,etc.The separation and purification methods of flavonoid compounds in Scutellaria baicalensis roots and aboveground parts are analyzed,such as column chromatography,high speed counter-current chromatography,etc.The composition determination methods of flavonoid compounds in Scutellaria baicalensis roots and aboveground parts are sorted out,including high performance liquid chromatography and ultra-high performance liquid chromatography,etc.We also propose suggestions for improving the practical application of Scutellaria baicalensis,such as combining laboratory research with industrial production,improving experimental equipment and extraction process to provide convenience for industrial production,making full use of Scutellaria baicalensis resources to create social benefits,etc.Based on the analysis of flavonoid compounds,this paper reviews the research progress of extraction,separation and determination methods of flavonoid compounds from Scutellaria baicalensis roots and aboveground parts,thus providing technical reference for the large-scale development and application of flavonoids from Scutellaria baicalensis and laying technical foundation for further research and promotion of Scutellaria baicalensis resources.

关 键 词：黄酮化合物 提取检测 黄芩 分离纯化 有机溶剂萃取法 柱层析色谱法 高效液相色谱法 

分 类 号：S63-33[农业科学—蔬菜学]