维氏气单胞菌TolC蛋白的生物信息学分析  

Bioinformatics analysis of Aeromonas veronii TolC protein

作　　者：李佳霓 李娟娟 马香[1] 唐燕琼[1] 刘柱[1] 李宏[1] LI Jiani;LI Juanjuan;MA Xiang;TANG Yanqiong;LIU Zhu;LI Hong(School of Life Sciences,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学生命科学学院,海南海口570228

出　　处：《中国病原生物学杂志》2023年第5期502-507,共6页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.32060153);海南省自然科学基金项目(No.322RC589)。

摘　　要：目的利用生物信息学技术预测和分析维氏气单胞菌TolC蛋白的生物学特性、结构、功能及进化关系,为研究其致病机制以及开发新的疫苗和新的药物靶点提供参考。方法从NCBI蛋白质数据库中获取TolC序列,使用ProtParam、ProtScale、SignalP5.0、TMHMM2.0、PSORTb、NetPhos3.1、CD-search、SPOMA、SWISS-MODEL、ABCpred、IEDB等生物信息学工具分析和预测TolC蛋白的理化性质、亲疏水性、跨膜区域、信号肽、保守结构域、亚细胞定位、磷酸化位点、二级和三级结构、B细胞抗原表位和互作蛋白。利用MEGA5.2构建系统进化树,分析不同细菌间TolC蛋白的进化关系。结果TolC蛋白由439个氨基酸组成,分子式为C_(2112)H_(3414)N_(600)O_(689)S_(5),是稳定的亲水性蛋白。该蛋白亚细胞定位于细胞外,无跨膜结构域,有信号肽SP(Sec/SPI),含有52个磷酸化位点和多个B细胞抗原表位。蛋白的主要二级结构为α-螺旋,三级结构为同源三聚体。TolC蛋白具有外膜通道蛋白的保守结构域,与B565_1337、macB等多药外排泵相关蛋白有互作关系。进化分析显示维氏气单胞菌TolC蛋白与嗜水气单胞菌TolC蛋白遗传进化关系最近。结论维氏气单胞菌TolC蛋白作为多药外排泵的外膜通道蛋白,与细菌致病和耐药性密切相关。该蛋白具有多个B细胞抗原表位,是潜在的药物靶点和疫苗候选蛋白。Objective To predict and analyze the biological characteristics,structure,function and evolutionary relationship of Aeromonas veronii TolC protein by bioinformatics technology,so as to provide reference for studying its pathogenic mechanism and developing new vaccines and new drug targets.Methods The sequence of TolC protein was obtained from NCBI protein database.ProtParam,ProtScale,SignalP5.0,TMHMM2.0,PSORTb,NetPhos3.1,CDsearch,SPOMA,SWISS-MODEL,ABCpred and IEDB were used to predict and analyze physicochemical properties,hydrophilicity,signal peptide,transmembrane domain,subcellular localization,phosphorylation sites,conserved domain,secondary structure,tertiary structure,B cell epitope and interacting proteins for TolC protein of A.veronii.MEGA5.2 was used to construct a phylogenetic tree to analyze the evolutionary relationship of TolC proteins among different bacteria.Results TolC protein consisted of 439 amino acids,the molecular formula was C_(2112)H_(3414)N_(600)O_(689)S_(5),and it was a stable hydrophilic protein.The protein was localized outside the cell,had signal peptide SP(Sec/SPI)but no transmembrane structural domain,and contained 52 phosphorylation sites as well as multiple B cell antigenic epitopes.Its secondary structure was mainlyα-helix,and the tertiary structure showed that it was homotrimer.TolC protein had the conserved domain of outer membrane channel protein and interacted with multiple drug efflux pump-related proteins such as B565_1337 and macB.Evolutionary analysis revealed that TolC protein of A.veronii was the closest to TolC protein of A.hydrophila.Conclusion As an outer membrane channel protein of multi-drug efflux pump,TolC protein is closely related to bacterial pathogenesis and drug resistance.The protein has multiple B cell antigenic epitopes,which can be used as a potential drug target and vaccine candidate protein.

关 键 词：维氏气单胞菌 外膜通道蛋白 TOLC 生物信息学 

分 类 号：R378[医药卫生—病原生物学]