m6A甲基化修饰在人类病毒感染复制中的作用  

The role of m6A methylation in human virus infection and replication

作　　者：彭倩[1] 乔嘉璐 李卫玲 孙宾莲 胡康洪[1] PENG Qian;QIAO Jialu;LI Weiling;SUN Binlian;HU Kanghong(Sino-German Biomedical Center,National“111”Center for Cellular Regulation and Molecular Pharmaceutics,Cooperative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province),Hubei University of Technology,Wuhan 430068,China;Wuhan Institute of Biomedical Sciences,School of Medicine,Jianghan University)

机构地区：[1]湖北工业大学中德生物医学中心,教育部及国家外专局细胞调控与分子药物学科“111”创新引智基地,教育部及湖北省工业发酵协同创新中心,湖北武汉430068 [2]江汉大学医学院,武汉生物医学研究院

出　　处：《中国病原生物学杂志》2023年第5期603-608,共6页Journal of Pathogen Biology

基　　金：湖北省重点研发计划项目(社会发展领域)(No.2022BCA018)。

摘　　要：真核细胞内RNA的m6A甲基化修饰通过调节基因的剪接、定位、稳定和翻译影响基因功能,其中甲基转移酶、去甲基化酶和阅读蛋白参与甲基化修饰过程。病毒感染引起传染性疾病,近年发现在病毒与宿主的博弈过程中,病毒RNA会受宿主m6A修饰系统的调控进而影响病毒复制,病毒蛋白通过影响宿主甲基化修饰相关调节因子从而影响整体修饰水平,改变细胞因子水平、干扰免疫细胞增殖和活性以及降低应激反应,以确保病毒顺利复制。本文概述了m6A甲基化修饰在甲型流感病毒(IAV)、人类I型免疫缺陷病毒(HIV-I)、新型冠状病毒(SARS-CoV-2)这三种典型的RNA病毒及乙型肝炎病毒(HBV)、Epstein-Barr病毒(EBV)、腺病毒(AdV)的DNA病毒感染复制中的作用。这些工作有助于探索靶向m6A调节因子的抑制剂,为开发新的抗病毒药物提供理论基础。The m6A methylation of RNA in eukaryotic cells affects gene function by regulating the splicing,localization,stabilization and translation of RNA,and methyltransferase,demethylase and reading proteins are involved in the methylation modification process.In recent years,it has been found that in the battle between virus and host,and host m6A modification system might affect viral replication by regulating viral RNA.In the same time,viral proteins also affect the overall m6A modification extent by influencing methylation modification-related factors to change the level of cytokines,interfere with the proliferation and the activity of immune cells,and reduce stress response,ultimately toensure that the virus replicates successfully.This article summarizes the roles of m6A modification during the infection and replication of three types of RNA viruses,including influenza A virus(IAV),human immunodeficiency virus type I(HIVI),and novel coronavirus(SARS-CoV-2),as well as three types of DNA viruses:hepatitis B virus(HBV),Epstein Barr virus(EBV),and adenovirus(AdV).These studies contribute to explore the inhibitors targeting m6A regulatory factors and will provide a theoretical clue toward the development of novel antiviral drugs.

关 键 词：m6A甲基化修饰 病毒感染 免疫应答 应激反应 抗病毒药物 综述 

分 类 号：R373[医药卫生—病原生物学]