不同群体日本沼虾的线粒体COI基因序列分析  

Analysis of mitochondrial COI sequences from different populations of Macrobrachium nipponense japonicus

作　　者：黄辉[1] 刘凯[1] 王宇希[1] 郭炜 马恒甲[1] 谢楠[1] HUANG Hui;LIU Kai;WANG Yu-xi;GUO Wei;MA Heng-jia;XIE Nan(Hangzhou Academy of Agricultural Sciences,Hangzhou310024,China)

机构地区：[1]杭州市农业科学研究院,杭州310024

出　　处：《湖北农业科学》2023年第5期179-182,189,共5页Hubei Agricultural Sciences

基　　金：杭州市农业科学研究院创新与推广基金项目(2021HNCT-16)。

摘　　要：采集钱塘江(QT)、武义(WY)、高塘湖(GT)、肇庆(ZQ)和南宁(NN)5个不同群体的日本沼虾(Macrobrachium nipponense)样本,并对每个群体的线粒体COI基因进行测序和分析,以此了解各群体间的遗传差异。结果表明,日本沼虾COI基因序列中,保守位点420个,变异位点222个,简约信息位点123个,碱基缺失或插入位点27个,平均转换与颠换比值2.78。COI基因序列中(A+T)的含量明显高于(G+C)的含量,碱基组成具有明显的AT偏倚性。在67个个体中定义了65种单倍型,来自NN群体和QT群体的单倍型形成了2个主要分支。基于遗传距离构建的系统发育树和聚类树表明,QT群体和GT群体遗传距离较近,与ZQ群体和NN群体相比遗传距离较远,WY群体与QT、GT群体的遗传距离较小。研究结果可为日本沼虾种质资源的合理开发利用提供分子生物学依据。Macrobrachium nipponense samples from five different populations including Qiantang River(QT),Wuyi(WY),Gaotanghu(GT),Zhaoqing(ZQ)and Nanning(NN)were collected in the experiment,and the mitochondrial COI gene of each population was sequenced and analyzed to understand the genetic differences among populations.The results showed that in the COI gene sequence of Macrobrachium nipponense,there were 420 conserved sites,222 mutated sites,123 reduced information sites,and 27 base deletion or insertion sites,with an average conversion to inversion ratio of 2.78.The content of(A+T)in the COI gene sequence was significantly higher than that of(G+C),and the base composition exhibited significant AT bias.65 haplotypes were defined in 67 individuals,and haplotypes from NN and QT populations formed two main branches.The phylogenetic tree and cluster tree constructed based on genetic distance showed that the genetic distance between QT population and GT population was relatively close,the genetic distance was relatively far compared with ZQ population and NN population,and the genetic distance between WY population and QT population and GT population was relatively small.The research results could provide a molecular biological basis for the rational development and utilization of Macrobracium germplasm resources.

关 键 词：日本沼虾(Macrobrachium nipponense) 线粒体 遗传差异 

分 类 号：S931.5[农业科学—渔业资源]