建兰种子无菌萌发及根状茎增殖分化研究  

Study on Aseptic Germination of Seeds and Rhizome’s Proliferation and Differentiation in Cymbidium ensifolium

作　　者：樊荣辉[1] 叶秀仙[1] 林榕燕 孔兰 林兵[1] 钟淮钦[1] Fan Ronghui;Ye Xiuxian;Lin Rongyan;Kong Lan;Lin Bing;Zhong Huaiqin(Institute of Crop Sciences,Fujian Academy of Agricultural Science/Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院作物研究所/福建省特色花卉工程技术研究中心,福建福州350013

出　　处：《东南园艺》2023年第1期7-10,共4页Southeast Horticulture

基　　金：福建省属公益科研院所基本科研专项(2020R1031001);闽台特色园林植物福建省高校重点实验室开放课题(MNNUMTTSYLZW-202011-2);福建省农业科学院科技创新团队项目(CXTD2021010-2);福建省农业科学院科技创新平台专项(CXPT202102);福建省种业创新与产业化工程项目(ZYCX-LY-202102)。

摘　　要：以建兰杂交种子为外植体,通过探索果荚采收时间、植物生长调节剂(6-BA、NAA)等关键因子对建兰种子萌发、根状茎增殖与分化的影响,探讨建兰种子无菌萌发及根状茎增殖与分化关键技术。结果表明:果荚最佳采收时期为花后8~10个月,种子萌发的适宜培养基为1/2MS+花宝1号1.5 g·L^(-1)+6-BA 2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+AC 0.3 g·L^(-1);根状茎增殖的适宜培养基为1/2MS+6-BA 0.5 mg·L^(-1)+NAA 1 mg·L^(-1)+AC 0.3 g·L^(-1),增殖系数达5.34;根状茎分化的适宜培养基为1/2MS+6-BA 2.0 mg·L^(-1)+NAA 0.3 mg·L^(-1)+AC 0.3 g·L^(-1),分化率达56%。Taking the hybrid seeds of Cymbidium ensifolium as explants,the key techniques of aseptic germination and rhizome’s proliferation and differentiation were discussed by exploring the effects of key factors such as harvest time of capsule and plant growth regulators(6-BA and NAA)on seed germination,rhizome proliferation and differentiation in Cymbidium ensifolium.The results showed that the best harvest time of capsule was 8-10 months after flowering,and the suitable medium for aseptic seed germination was 1/2MS+Hyponex No.1 medium 1.5 g·L^(-1)+6-BA 2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+AC 0.3 g·L^(-1);the suitable proliferation medium for rhizomes was 1/2MS+6-BA 0.5 mg·L^(-1)+NAA 1 mg·L^(-1)+AC 0.3 g·L^(-1),and the proliferation coefficient reached 5.34;the suitable differentiation medium for rhizomes was 1/2MS+6-BA 2.0 mg·L^(-1)+NAA 0.3 mg·L^(-1)+AC 0.3 g·L^(-1),and the differentiation rate was 56%.

关 键 词：建兰 无菌萌发 根状茎 增殖 分化 

分 类 号：S682.31[农业科学—观赏园艺]