64个蓝莓品种的ISSR分析  

ISSR Analysis of 64 Blueberry Cultivars

作　　者：张立杰[1] 谢丽雪[1] 张小艳[1] 李韬[1] 黄立新 Zhang Lijie;Xie Lixue;Zhang Xiaoyan;Li Tao;Huang Lixin(Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Shaowu Municipal Bureau of Agriculture and Rural Affairs,Nanping,Fujian 354000,China)

机构地区：[1]福建省农业科学院果树研究所,福建福州350013 [2]邵武市农业农村局,福建南平354000

出　　处：《东南园艺》2023年第1期11-17,共7页Southeast Horticulture

基　　金：福建省属公益类科研院所基本科研专项(2020R10280011、2019R1028-1)。

摘　　要：为明确不同蓝莓品种间的遗传关系,利用ISSR标记对64个蓝莓品种进行DNA指纹分析,构建蓝莓品种的指纹图谱数据库。从60条ISSR引物中筛选出9条重复性稳定的多态性引物,共扩增出条带161条,其中多态性条带159条,平均多态百分率达98.8%,引物UBC841可将64个蓝莓品种区分开。利用扩增结果进行聚类分析,在相似系数0.568的水平可将试材分为3个类群,并对其遗传关系进行探讨。In order to clarify genetic relationship of different cultivars of blueberry,the inter-simple sequence repeat(ISSR)method was applied to analyze the DNA fingerprinting of 64 blueberry cultivars,and a fingerprints database was constructed.Nine ISSR primers,with high polymorphisms and good repeatability,were selected from 60 ISSR primers.Total 161 DNA bands were amplified,159 of which were polymorphic,the average polymorphism percentage was 98.8%.These varieties could be satisfactorily distinguished by a single ISSR primer UBC841.UPGMA algorithm showed that 64 cultivars could be clustered into 3 groups using a genetic similarity coefficient threshold of 0.568,and their genetic relationships was evaluated.

关 键 词：蓝莓 品种 ISSR 指纹图谱 遗传关系 

分 类 号：S682.31[农业科学—观赏园艺]