高效液相色谱测定丙氨酰谷氨酰胺中邻苯二甲酰肼残留  

Determination of Phthalhydrazide Residues in Alanyl-glutamine by HPLC

作　　者：汪华 陈鹏 王涛 刘晓宇 钟旭斌 Wang Hua;Chen Peng;Wang Tao;Liu Xiaoyu;Zhong Xubin(Livzon Syntpharm Co.,Ltd.,(Zhuhai FTZ),Guangdong Zhuhai 519030;Institute of Teacher Education,Taishan University,Tai an,271021,China)

机构地区：[1]珠海保税区丽珠合成制药有限公司,广东珠海519030 [2]泰山学院教师教育研究院,山东泰安271021

出　　处：《云南化工》2023年第5期85-87,共3页Yunnan Chemical Technology

摘　　要：为了准确测定出丙氨酰谷氨酰胺中邻苯二甲酰肼残留量,利用高效液相色谱法,通过对色谱体系、流动性比例、色谱柱及柱温、波长进行选择,确定了最优的色谱条件。同时,为了验证该方法的可行性,进行方法学验证实验,通过测定专属性、精密度、线性与范围、溶液稳定性等实验,结果表明,该方法对于测定丙氨酰谷氨酰胺中邻苯二甲酰肼残留具有可行性。In order to accurately determine the residual amount of phthalhydrazide in alanyl-glutamine,we determined the optimal chromatographic conditions by using high performance liquid chromatography through the selection of chromatographic system,mobility ratio,chromatographic column,column temperature and wavelength.At the same time,in order to verify the feasibility of this method,we carried out a methodology validation experiment.Through the determination of specificity,precision,linearity and range,solution stability and other experiments,the experimental results showed that this method was feasible for the determination of phthalhydrazide residues in alanyl-glutamine.

关 键 词：丙氨酰谷氨酰胺 邻苯二甲酰肼 高效液相色谱 

分 类 号：X832[环境科学与工程—环境工程]