条石鲷(Oplegnathus fasciatus)雌核发育的人工诱导与鉴定  

ARTIFICIAL INDUCTION AND IDENTIFICATION OF DIPLOID GYNOGENESIS IN OPLEGNATHUS FASCIATUS

作　　者：陈睿毅 刘明涛 竺奇慧 李海东 胡伟华 徐冬冬[1,2] CHEN Rui-Yi;LIU Ming-Tao;ZHU Qi-Hui;LI Hai-Dong;HU Wei-Hua;XU Dong-Dong(Zhejiang Marine Fisheries Research Institute,Zhoushan 316021,China;Key Lab of Mariculture and Enhancement of Zhejiang Province,Zhoushan 316021,China;School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江省海洋水产研究所,浙江舟山316021 [2]浙江省海水增养殖重点实验室,浙江舟山316021 [3]浙江海洋大学水产学院,浙江舟山316022

出　　处：《海洋与湖沼》2023年第3期829-835,共7页Oceanologia Et Limnologia Sinica

基　　金：国家重点研发计划,2019YFD0901204号;浙江省自然科学基金,LQ20C190008号;浙江省海水增养殖重点实验室开放课题,2021KF002号。

摘　　要：条石鲷(Oplegnathus fasciatus)是我国重要的经济鱼类,具有复性染色体性别决定型(X_1X_1X_2X_2/X_1X_2Y)。利用紫外线(UV)灭活的同源精子通过冷休克方法诱导了条石鲷的雌核发育,筛选精子紫外灭活剂量并优化冷休克相关参数。结果表明:条石鲷精子最适的灭活剂量为80 mJ/cm2雌核发育的最适条件为授精后2 min在3~4℃水温下处理10 min。条石鲷雌核发育子代经形态学和流式细胞仪鉴定为二倍体,进一步利用微卫星分析表明雌核发育后代的等位基因完全来自于母本没有父本基因参与。对子代的性比和生长进行了比较,结果表明条石鲷雌核发育后代均为雌性,15月龄后雌核发育后代比普通条石鲷具有更快的生长速度。研究结果为条石鲷单性苗种的培育及其性别决定机制的深入研究提供基础。The rock bream(Oplegnathus fasciatus)is a commercially important fish species in China,and it has a multiple X1X1X2X2/X1X2Y sex chromosome system.The induction of diploid gynogenesis in O.fasciatus was established using a combination of UV-irradiated homologous sperm and cold shock of the resulting embryos to inhibit the second meiotic division.The optimal UV irradiation of the O.fasciatus sperm for gynogenesis was 80 mJ/cm2.The treatment optima for diploid gynogenesis were 3~4℃for 10 min at 2 min after fertilization.Results obtained from the gonad morphological observation and larvae DNA content detection by flow cytometry proved that the gynogenesis-induced larvae were diploid gynogenesis.Furthermore,the genetic analysis revealed exclusively the maternal inheritance in all gynogenetic fry,and no paternal contributions were detected.The sex ratio and growth performance were compared in two offsprings,and all the gynogenetic offspring were female,showing faster growth than normal O.fasciatus after 15 month post hatching.This was the first protocol of induced gynogenesis in Oplegnathidae fish;and this study lay a basis for the production of all-female populations in a large scale,and clarified the sex determination mechanisms in the fish.

关 键 词：条石鲷(Oplegnathus fasciatus) 雌核发育 冷休克 微卫星 复性染色体 

分 类 号：Q955[生物学—动物学] Q789[农业科学—水产养殖] S965.325[农业科学—水产科学]