蝴蝶豆种质资源芽期耐盐性筛选及评价  

Screening and Evaluation of Salt Tolerance in the Bud Stage of Centrosema pubescens Resources

作　　者：王燕茹 袁秉琛 孙郁婷 王志勇[1] 虞道耿[3] WANG Yanru;YUAN Bingchen;SUN Yuting;WANG Zhiyong;YU Daogeng(College of Forestry,Hainan University,Haikou,Hainan 570228,China;College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学林学院,海南海口570228 [2]海南大学热带作物学院,海南海口570228 [3]中国热带农业科学院热带作物品种资源研究所,海南海口571101

出　　处：《热带作物学报》2023年第5期955-967,共13页Chinese Journal of Tropical Crops

基　　金：海南省农业农村厅物种资源保护项目“海南饲用豆科植物资源保护”(琼财农[2020]829号);果园间作牧草技术模式研究与示范(No.PZS2022001)。

摘　　要：蝴蝶豆作为重要的热带豆科牧草,研究其耐盐机理,对选育优良的品种和提高牧草的产量具有十分重要的意义。为研究不同蝴蝶豆(Centrosema pubescens)种子萌发期耐盐性差异,以150 mmol/L浓度的氯化钠对75份蝴蝶豆属种质在萌芽期进行耐盐性鉴定,测定发芽率、发芽势、根长、芽长和鲜重等14个指标,并进行隶属函数、主成分分析、相关性分析以及聚类分析,从而对蝴蝶豆种质资源耐盐性进行综合评价,筛选耐盐种质。结果表明:盐胁迫浓度在150 mmol/L下,相对根冠比变异系数最大(67.75%),可以作为蝴蝶豆耐盐性评价指标之一;相关性分析展现出蝴蝶豆属种质发芽率、发芽势相关任意2个发芽指标之间呈现极显著(P<0.01)正相关,其余各类指标相互均存在一定程度的关联性;本研究中提取了4个主成分作为主成分分析的方向,累积贡献率83.17%,可以代替所有指标的大部分信息,综合反映盐胁迫下蝴蝶豆萌芽期的萌发特性,并通过载荷矩阵确定发芽指数、相对根冠比、相对发芽率和根冠比为耐盐性评价指标;对平均隶属函数和综合得分进行耐盐性综合评价,并基于蝴蝶豆耐盐综合得分值(Z)进行聚类,将75份种质资源分为4个大类,其中极耐盐种质1份(050321057蝴蝶豆)、耐盐种质17份、低耐盐种质7份、敏盐种质共50份。本研究建立的蝴蝶豆耐盐评价体系和筛选方法可为蝴蝶豆属耐盐性、耐盐基因筛选等研究提供基础。Centromea pubescens is an important tropical leguminous forage.It is of great significance to study the salt tolerance mechanism of the plant for breeding excellent varieties and improving forage yield.In order to study the dif-ference of salt tolerance of different seeds of C.pubescens in the germination period,the salt tolerance of 75 germplasms was identified in the germination period.NaCl with 150 mmol/L,and 14 indexes such as germination rate,germination potential,root length,bud length and fresh weight were measured,and membership function,principal component analysis,correlation analysis and cluster analysis were carried out to comprehensively evaluate the salt tolerance of germplasm resources of pteromea and to screening salt tolerant germplasm.Under salt stress concentration of 150 mmol/L,the coefficient of variation of relative root shoot ratio was the highest(67.75%),which could be used as one of the evaluation indicators of salt tolerance.The correlation analysis showed that there was a very significant(P<0.01)relationship between any two germination indicators of the germination rate and germination potential,and the other indicators were related to each other to a certain extent.Four principal component as the main component analysis of the direction of the cumulative contribution rate of 83.17%could replace most of the information of all indicators,comprehensively reflecting the germination characteristics during germination under salt stress,and determine the germination index,relative root shoot ratio,relative germination rate and root shoot ratio as the evaluation indicators of salt tolerance through the load matrix.The average membership function and comprehensive score were comprehensively evaluated for salt tolerance,and the 75 germplasm resources were divided into four categories by clustering based on the comprehensive score value of salt tolerance(Z),including 1 highly salt tolerant germplasm(Centrosema 050321057),17 salt tolerant germplasm,7 low salt tolerant germplasm and 50 salt sensiti

关 键 词：蝴蝶豆 相关性分析 主成分分析 隶属函数 聚类分析 

分 类 号：S541.9[农业科学—作物学]