南药巴戟天脱毒苗的培育与快繁体系的优化  

Cultivation of Detoxification Seedlings and Optimization of the Rapid Propagation System of Southern Medicine Morinda officinalis How

作　　者：陈子恩 冯冲 罗振华 刘梦云 丁平[1] CHEN Zi’en;FENG Chong;LUO Zhenhua;LIU Mengyun;DING Ping(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006,China;Guangzhou Caizhilin Pharmaceutical Co.,Ltd.,Guangzhou,Guangdong 510000,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州采芝林药业有限公司,广东广州510000

出　　处：《热带作物学报》2023年第5期968-976,共9页Chinese Journal of Tropical Crops

基　　金：广东省农业科技创新及推广体系建设项目(No.2021KJ142);国家重点研发计划项目(No.2017YFC1701102);2021年广东省级乡村振兴战略专项(No.2021KJ268)。

摘　　要：巴戟天(Morinda officinalis How)为茜草科巴戟属植物,其干燥根入药,能够强健筋骨、滋补肾阳、祛风除湿,是中国四大南药之一,在我国主产区主要通过扦插繁殖,品种选育过程中往往受到植物病毒的干扰,造成植物生长受到抑制、产量及品质下降。因此,以巴戟天的组织培养脱毒为目的,对巴戟天进行组织培养脱毒研究,从而提高巴戟天的产量及质量,对保护巴戟天种质资源及巴戟天的工厂化育苗提供技术支撑,具有重要的现实意义。利用巴戟天茎段为外植体,建立高效、稳定的巴戟天组织快繁体系,筛选出适宜的植株再生途径。以1/2 MS为基础培养基,利用巴戟天茎段腋芽诱导丛生芽获得巴戟天的脱毒苗并成功驯化移栽,通过PCR技术对巴戟天组培苗与移栽苗进行病毒检测。结果表明,以巴戟天半木质化茎段作为外植体诱导形成腋芽最优,最适培养基为1/2 MS+6-BA 0.2 mg/L,腋芽诱导率为70%;以巴戟天腋芽诱导丛生芽,最适培养基为1/2 MS+6-BA 0.2 mg/L,丛生芽诱导率达到86.36%,生长状况良好;适宜生根的培养基为1/2 MS+IBA 0.5 mg/L,生根率达100%;组培苗移栽于草炭土-珍珠岩1∶1的基质中炼苗8 d成活率最高,达到93.3%;经过反转录PCR检测巴戟天组培苗与移栽苗中黄瓜花叶病毒-巴戟天株(Cucumber mosaic virus isolate Morinda officinalis How,CMVMO),结果显示组培苗成功脱毒。本研究通过直接器官发生途径,成功培育出巴戟天脱毒苗,建立了巴戟天体外快繁再生体系,不仅提高了巴戟天的产量和品质,为南药巴戟天种苗的脱毒快繁及商业化生产育苗提供参考。Morinda officinalis How is a plant of the Rubiaceae,whose dried roots are used as medicine It can strengthen bones and muscles,nourish kidney-yang and remove wind and moisture.It is mainly distributed in Guangdong,Guangxi,Fujian and Hainan provinces.It is one of the four major southern medicines in China.In the main production areas of China,it is mainly propagated by cuttings.In the process of variety breeding,it is often disturbed by plant viruses.The viruses are transmitted and accumulated in plants through asexual propagation of plants,resulting in the inhibition of plant growth and the decline of yield and quality.Therefore,for the purpose of tissue culture detoxification of M.officinalis,the tissue culture detoxification research of M.officinalis was carried out.Thereby improving the yield and quality of M.officinalis,which is beneficial to the protection of M.officinalis species.It is of great practical significance to provide technical support for quality resources and the factory-based seedlings of M.officinalis.Using the stem segments as the explants,an efficient and stable M.officinalis tissue rapid propagation system was established,and suitable plant regeneration methods were screened out.Using 1/2MS as the basic medium,M.officinalis stem axillary buds were used to induce clustered buds to obtain the virus-free seedlings and successfully domesticated and transplanted,the virus detection of M.officinalis tissue culture seedlings and transplanted seedlings were carried out by PCR technology.The semi-lignified stem segments as the explants were suitable to induce axillary buds,the most suitable medium was 1/2MS+6-BA 0.2 mg/L,and the axillary bud induction rate was 70%.Taking the axillary buds to induce fascicled bud,the most suitable medium was 1/2MS+6-BA 0.2 mg/L,and the fascicled bud induction rate was 86.36%.The suitable medium for rooting was 1/2MS+IBA 0.5 mg/L,with a root rate of 100%.The tissue culture seedlings were transplanted in the 1∶1 peat soil-perlite matrix for 8 days with the highest su

关 键 词：巴戟天 脱毒 快繁 直接器官发生 病毒检测 

分 类 号：R285[医药卫生—中药学]