桑小头木虱内生殖系统的形态特征及其在不同发育时期的变化趋势研究  

Morphological Characteristics and the Change Trends of the Internal Reproductive System of Paurocephala sauteri Adults in Different Developmental Stage

作　　者：韦红显 耿涛 武华周 娄德钊 吴春标 谢云会 卢芙萍[2] 王树昌[2] WEI Hongxian;GENG Tao;WU Huazhou;LOU Dezhao;WU Chunbiao;XIE Yunhui;LU Fuping;WANG Shuchang(Hainan University,Haikou,Hainan 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture&Rural Affairs/Hainan Provincial Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Haikou,Hainan 571101,China;Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]海南大学,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物病虫害综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海南海口571101 [3]贵州大学,贵州贵阳550025

出　　处：《热带作物学报》2023年第5期1023-1029,共7页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.320RC689);现代农业产业技术体系专项资金项目(No.CARS-18-SYZ17);海南省重点研发计划项目(No.ZDYF2020219)。

摘　　要：桑小头木虱是近年来热带亚热带地区为害桑树的最重要害虫,个体小,繁殖快,世代重叠,导致桑叶产量损失超40%,苗期受害甚至绝收,防治难度大。为准确预测和高效防治桑小头木虱,提高桑叶产量和质量,本研究对桑小头木虱不同发育时期雌雄成虫内生殖系统进行解剖和显微观察,初步明确其雌雄成虫内生殖系统形态特征及其在交配前后的变化趋势。结果表明,桑小头木虱雌性内生殖系统组成除侧输卵管有2对外,与非洲柑橘木虱相似;雄性内生殖系统组成则与亚洲柑橘木虱高度相似。与交配前期相比,雌成虫受精囊的长度在交配期和交配后期分别达交配前期的约1.63倍和1.85倍,宽度约增加至交配前期的1.74倍和1.84倍,各时期存在显著差异(P<0.05),卵粒的长度和宽度均快速增加,至交配期增加近2倍(P<0.05),同时,交配期卵粒内部可见数个体积较大的囊泡,至交配后期消失,其应为滋养细胞,为卵母细胞提供营养。与交配前期相比,雄成虫精巢在交配期增至最大,交配后期缩至最小,3个时期存在显著差异(P<0.05),而在交配期和交配后期,贮精囊的长度和宽度均显著增加,增至2倍左右(P<0.05)。本研究对准确判断并确定桑小头木虱防治适期、生殖相关基因的筛选和新型基因靶向药剂的研发应用,以及高效监测与防控措施的制定具有现实意义。Paurocephala sauteri,characterized by small individuals,rapid reproduction and overlapped generations,is the most damaging pest of mulberry trees in tropical and subtropical areas.It happens in seedling period,leading to yield loss over 40%,or even out of harvest,and is very difficult to control.In order to accurately predict and efficiently control the pest,and to improve the yield and quality of mulberry leaves,the internal reproductive system of the male and female adults of the pest in different developmental periods was dissected and observed to clarify the morphological characteristics and changes of the internal reproductive system of male and female adults of the pest before and after mating.Except for 2 pairs of lateral oviducts the composition of the internal reproductive system of the female adults is similar to Trioza erytreae(Del guercio),and male adults is highly similar to Diaphorina citri(Kuwayama).Compared with the pre-mating period,the length of spermatheca in mating and post-mating periods reached nearly 1.63 times and 1.85 times,and the width increased by about 1.74 times and 1.84 times respectively,with significant differences in each period(P<0.05),the length and width of eggs increased rapidly,to mating period increased by nearly 2 times(P<0.05).At the same time,several large vesicles appeared inside the egg in mating period and disappeared in post-mating period,them should be trophoblasts,providing nutrition for oocytes.Compared with the pre-mating period,the testes of the male increased to the maximum in the mating period and shrank to the minimum in the post-mating period.There were significant differences in the three periods(P<0.05),while the length and width of the seminal vesicle both significantly increased to about twice(P<0.05)in mating and post-mating periods.This study has practical significance to precisely judge and determine the appropriate period of prevention and control,to clone reproductive-related genes,to develop new gene targeting agents,and to formulate efficient prev

关 键 词：桑小头木虱 内生殖系统 形态特征 变化趋势 

分 类 号：S888.723[农业科学—特种经济动物饲养]