天然血管基质/聚己内酯复合血管支架的制备与性能表征  

Preparation and properties characterization of natural vascular matrix/polycaprolactone composite vascular scaffolds

作　　者：陈国宝 董刚立 杨欢 陈忠敏 王富平 CHEN Guobao;DONG Gangli;YANG Huan;CHEN Zhongmin;WANG Fuping(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《重庆理工大学学报（自然科学）》2023年第5期308-317,共10页Journal of Chongqing University of Technology：Natural Science

基　　金：重庆市自然科学基金项目(cstc2020jcyj-msxmX0350)。

摘　　要：将脱细胞血管基质(decellularized vascular matrix,DVM)与聚己内酯(Polycaprolactone,PCL)相复合,得到的复合支架能保留天然血管基质的血管诱导再生特性,克服降解过快的不足,扩大其在组织工程领域的应用范围。将新鲜的猪主动脉经脱细胞、脱脂等一系列处理后得到DVM粉末,再将其经酶解得到脱细胞血管基质凝胶(decellularized vascular matrix gel,DVMG),将用冰醋酸配置的PCL溶液与DVMG按照不同比例混合,将混合溶液反复包裹模具,室温中冷却后得到不同组的复合血管支架。共制备出4组DVMG/PCL的支架材料,通过扫描电子显微镜(scanning electron microscope,SEM)观察发现支架表面较为均匀,截面结构有明显孔隙,利于细胞黏附与迁移。通过力学拉伸实验检测PCL支架、DVMG∶PCL分别为1∶1,1.5∶1,5∶1的各组支架的拉伸模量值依次为49.00±9.52 MPa,46.29±3.08 MPa,38.77±2.07 MPa和31.18±2.80 MPa。体外降解实验显示:4组支架材料均有一定的降解能力,降解速率与DVMG的含量有关,说明改变DVMG与PCL的比例可以制备出不同力学性能、降解性能的复合血管支架。相较于单纯的PCL支架,复合支架中DVMG的含量越高,支架的刚度越低,降解速率越快,DVMG/PCL支架的整体性能得到一定改善。研究结果对于进一步扩大DVM和DVMG的应用范围,改善高分子材料的生物学响应性能具有参考价值。Vascular diseases are one of the leading causes of death in China and even in the global population.Currently,common vascular grafts can be broadly divided into autologous vascular grafts,xenogeneic vascular grafts as well as vascular tissue engineering.Autografts are the gold standard in a clinical surgery,but they are unfavorable for the subsequent treatment of patients because of repeated surgeries and poor sources.In addition,easily producing rejection reaction with the body to cause damages due to poor biocompatibility,xenogeneic vascular transplantation not only has immunogenicity,but may also spread diseases to cause infection.Therefore,it is difficult to meet the requirements of vascular transplantation.However,the rapid development of tissue engineering technology provides a new solution for the treatment of vascular diseases.At present,all the vascular scaffolds developed at home and abroad have their advantages and disadvantages,which often require multiple composite materials to achieve advantage complementation.Traditional decellularized scaffolds may have lower cell permeability due to their dense extracellular matrix network.Recently,it has been shown that decellularized vascular matrix gel(DVMG)is a promising material that not only has the advantage of decellularized vascular matrix(DVM),but can also control the mechanical properties of vascular scaffolds by changing the concentration or cross-linking density of hydrogels.This study complexes DVMG with polycaprolactone(PCL),and the resulting composite scaffolds can retain the vascular regenerative properties of the native vascular matrix and overcome the deficiency its rapid degradation,expanding its applications in the field of tissue engineering.DVM powder is obtained through decellularization and delipidation of fresh porcine aorta in a series of treatments,after which the DVMG is enzymatically dissociated,the PCL solution configured with glacial acetic acid is mixed with DVMG with different proportions,and the molds are repeatedly wrapped by t

关 键 词：血管支架 脱细胞血管基质 聚己内酯 力学特征 组织工程 

分 类 号：Q819[生物学—生物工程]