GnT-IVa对京尼平苷调节胰腺β细胞GLUT2糖基化及葡萄糖摄取的影响  

Effect of GnT-IVa on the regulation of geniposide in the glycosylation of glucose transporter 2 and glucose uptake in pancreatic beta cells

作　　者：李维钊 申甚莉 边阳萍 赵婉均 汪夏 殷菲[1,2] LI Weizhao;SHEN Shenli;BIAN Yangping;ZHAO Wanjun;WANG Xia;YIN Fei(Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China;School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学药物化学与分子药理学重庆市重点实验室,重庆400054 [2]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《重庆理工大学学报（自然科学）》2023年第5期324-330,共7页Journal of Chongqing University of Technology：Natural Science

基　　金：重庆市科技计划重点项目(cstc2017jcyjBX0077);重庆市高校优秀创新团队项目(CXTDX201601031)。

摘　　要：N-乙酰氨基葡萄糖转移酶-4a(N-acetylglucosamine transferase-4a,GnT-IVa)是胰岛β细胞中葡萄糖转运蛋白2(glucose transporter 2,GLUT2)形成N-糖链分支复合物、使其驻留在细胞膜表面完成正常生理功能所必需的糖基转移酶。课题组前期研究发现,京尼平苷可快速调节胰腺β细胞中GLUT2糖基化和葡萄糖摄取,但GnT-IVa是否与其有直接的联系,还有待研究。首先利用siRNA干扰INS-1细胞GnT-IVa表达,随后分析京尼平苷对GLUT2糖基化、葡萄糖摄取、代谢以及葡萄糖诱导胰岛素分泌(glucose-stimulated insulin secretion,GSIS)的影响。结果证实,在GnT-IVa干扰的INS-1细胞中,京尼平苷对GLUT2糖基化的影响、葡萄糖吸收、ATP生成以及GSIS被明显抑制。研究结果提示,GnT-IVa可能在京尼平苷调节胰岛β细胞GLUT2糖基化、葡萄糖摄取、代谢和GSIS过程中有重要作用。N-acetylglucosamine transferase-4a(GnT-IVa)is an indispensable glycosyltransferase for the formation of N-glycan branch complex of glucose transporter 2(GLUT2)in pancreatic beta cells,which sits the N-glycan branch complex on the surface of cell membranes to perform its normal physiological functions.The previous work of this research group indicates that geniposide can rapidly regulate the glycosylation of GLUT2 and glucose uptake in pancreatic beta cells,but whether GnT-IVa is directly related to it is still not clear.This paper firstly uses siRNA to interfere GnT-IVa expression of INS-1 cells,and then analyzes the effect of geniposide on the glycosylation of GLUT2,glucose uptake and metabolism,and glucose-stimulated insulin secretion(GSIS).The results reveal that,in INS-1 cells interfered by GnT-IVa genes,the effects of geniposide on the glycosylation of GLUT2,glucose uptake,ATP production and GSIS are significantly attenuated.All these findings indicate that GnT-IVa may play an important role in geniposide regulating the glycosylation of GLUT2,glucose uptake and metabolism,and GSIS in pancreatic beta cells.

关 键 词：京尼平苷 N-乙酰氨基葡萄糖转移酶-4a 糖基化 葡萄糖摄取 

分 类 号：R977[医药卫生—药品]