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作 者:杨东[1,2] 骆名瑞 石义涛 谢兰宇 YANG Dong;LUO Ming-rui;SHI Yi-tao;XIE Lan-yu(Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350018,China;Life Science and Technology Center,China Seed Group Co.,Ltd.,Wuhan,Hubei 430206,China)
机构地区:[1]福建省农业科学院水稻研究所,福建福州350018 [2]中国种子集团有限公司生命科学技术中心,湖北武汉430206
出 处:《福建农业科技》2023年第3期56-61,共6页Fujian Agricultural Science and Technology
基 金:福建省农业科学院对外合作项目(DWHZ-2022-13);福建省科技计划公益类项目(2021R1023004);福建省种业创新与产业化工程项目(fjzycxny2021001)。
摘 要:除草剂的使用会降低水稻的产量和品质,开展非转基因的抗除草剂水稻品种改良迫在眉睫。以中粳616(受体材料)及抗除草剂品种金粳818、净田美占为试验材料,经除草剂(先达豆施乐,5%咪唑乙烟酸含量)浓度梯度试验筛选抗除草剂处理浓度,通过杂交、回交、自交结合除草剂耐药性筛选获得中粳616-TR,进一步分析除草剂对改良后中粳616-TR幼苗萌发及生长的影响,同时以中粳616为对照进行品比试验。结果表明:除草剂浓度梯度试验结果显示,可将3 mL·L^(-1)作为后续抗除草剂水稻筛选浓度。改良获得的中粳616-TR在3 mL·L^(-1)浓度除草剂处理下,在萌发期和苗期对除草剂均有耐药性,品比试验发现中粳616-TR在农艺性状、产量、抗病性、品质方面与中粳616无显著差异。综上,改良后的中粳616-TR具有品质优、稻瘟病抗性好、产量高兼备抗除草剂性状等特点。The use of herbicides would reduce the yield and quality of rice.It was urgent to improve the non-transgenic herbicide-tolerant rice varieties.Zhongjing 616(receptor material)and the herbicide-tolerant varieties,such as Jinjing 818 and Jingtian Meizhan,were selected as the experimental materials,and the concentration gradient test of herbicide(Xianda Doushile,5%imidazoic acid content)was used to screen the herbicide-tolerant treatment concentrations.Zhongjing 616-TR was obtained through the hybridization,backcrossing and self-crossing combined with the screening of herbicide resistance.The effects of herbicides on the seedling germination and growth of the improved Zhongjing 616-TR were further analyzed.At the same time,Zhongjing 616 was used as the control group to carry out the variety comparison test.The results showed that the herbicide concentration gradient experimentation results showed that 3 mL·L^(-1) could be used as the follow-up screening concentration of herbicide-tolerant rice.The improved Zhongjing 616-TR was resistant to herbicides at the germination stage and the seedling stage when treated with 3 mL·L^(-1)herbicide.The variety comparison test showed that there was no significant difference in the agronomic traits,yield,disease resistance and quality between Zhongjing 616-TR and Zhongjing 616.In summary,the improved Zhongjing 616-TR had the characteristics of excellent quality,good rice blast resistance,high yield and herbicide resistance.
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