Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice  

作　　者：Siliang Xu Mengchen Zhang Junhua Ye Dongxiu Hu Yuanyuan Zhang Zhen Li Junrong Liu Yanfei Sun Shan Wang Xiaoping Yuan Yue Feng Qun Xu Xinghua Wei Dali Zeng Yaolong Yang 

机构地区：[1]China National Center for Rice Improvement/State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,Zhejiang,China [2]The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China

出　　处：《The Crop Journal》2023年第3期733-743,共11页作物学报（英文版）

基　　金：supported by the Key Research and Development Program of Zhejiang Province(2021C02056,2021C02063-6);the National Key Research and Development Program of China(2021YFD1200503);the Fundamental Research Funds for Central Public Welfare Research Institutes of China National Rice Research Institute(CPSIBRF-CNRRI-202101);Chinese Academy of Agricultural Sciences(CAAS-ASTIP-201X-CNRRI)。

摘　　要：Because plant mechanical strength influences plant growth and development,the regulatory mechanisms underlying cell-wall synthesis deserve investigation.Rice mutants are useful for such research.We have identified a novel brittle culm 25(bc25)mutant with reduced growth and partial sterility.BC25 encodes an UDP-glucuronic acid decarboxylase involved in cellulose synthesis and belongs to the UXS family.A single-nucleotide mutation in BC25 accounts for its altered cell morphology and cellwall composition.Transmission electron microscopy analysis showed that the thickness of the secondary cell wall was reduced in bc25.Monosaccharide analysis revealed significant increases in content of rhamnose and arabinose but not of other monosaccharides,indicating that BC25 was involved in xylose synthesis with some level of functional redundancy.Enzymatic assays suggested that BC25 functions with high activity to interconvert UDP-glucuronic acid(UDP-Glc A)and UDP-xylose.GUS staining showed that BC25 was ubiquitously expressed with higher expression in culm,root and sheath,in agreement with that shown by quantitative real-time(q RT)-PCR.RNA-seq further suggested that BC25 is involved in sugar metabolism.We conclude that BC25 strongly influences rice cell wall formation.

关 键 词：Brittle culm Mechanical strength UDP-xylose Cell wall RICE 

分 类 号：S511[农业科学—作物学]