Flavanone and flavonoid hydroxylase genes regulate fiber color formation in naturally colored cotton  

作　　者：Hongli Zheng Bailin Duan Bo Yuan Zhengbin Chen Dongliang Yu Liping Ke Wenlong Zhou Haifeng Liu Yuqiang Sun 

机构地区：[1]Plant Genomics and Molecular Improvement of Colored Fiber Laboratory,College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,Zhejiang,China [2]College of Textile Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,Zhejiang,China [3]China Colored-cotton(Group)Co.,Ltd.,Urumqi 830011,Xinjiang,China

出　　处：《The Crop Journal》2023年第3期766-773,共8页作物学报（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang Province(LZ21C130004);the National Natural Science Foundation of China(U1903204);he Fundamental Research Funds of Shaoxing Keqiao Research Institute of Zhejiang Sci-Tech University(KYY2021004S)。

摘　　要：Using naturally colored cotton(NCC)can eliminate dyeing,printing and industrial processing,and reduce sewage discharge and energy consumption.Proanthocyanidins(PAs),the primary coloration components in brown fibers,are polyphenols formed by oligomers or polymers of flavan-3-ol units derived from anthocyanidins.Three essential structural genes for flavanone and flavonoid hydroxylation encoding flavanone-3-hydroxylase(F3H),flavonoid 3’-hydroxylase(F3’H)and flavonoid 3’5’-hydroxylase(F3’5’H)are initially committed in the flavonoid biosynthesis pathway to produce common precursors.The three genes were all expressed predominantly in developing fibers of NCCs,and their expression patterns varied temporally and spatially among NCC varieties.In GhF3Hi,GhF3’Hi and GhF3’5’Hi silenced lines of NCC varieties XC20 and ZX1,the expression level of the three genes decreased in developing cotton fiber,negatively correlated with anthocyanidin content and fiber color depth.Fiber color depth and type in RNAi lines changed with endogenous gene silencing efficiency and expression pattern,the three hydroxylase genes functioned in fiber color formation.GhF3H showed functional differentiation among NCC varieties and GhF3’H acted in the accumulation of anthocyanin in fiber.Compared with GhF3’H,GhF3’5’H was expressed more highly in brown fiber with a longer duration of expression and caused lighter color of fibers in GhF3’5’H silenced lines.These three genes regulating fiber color depth and type could be used to improve these traits by genetic manipulation.

关 键 词：Naturally colored cotton Brown cotton fiber Fiber color Anthocyanidin Flavanone/flavanoid hydroxylase 

分 类 号：S562[农业科学—作物学]