Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency  

作　　者：Jie Luo Junnan Hang Bilong Wu Xilin Wei Quanzhi Zhao Zhongming Fang 

机构地区：[1]Institute of Rice Industry Technology Research,Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Agricultural Sciences,Guizhou University,Guiyang 550025,Guizhou,China [2]Center of Applied Biotechnology,Wuhan University of Bioengineering,Wuhan 430415,Hubei,China

出　　处：《The Crop Journal》2023年第3期785-799,共15页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32260498);the Guizhou Provincial Science and Technology Project(qiankehejichu-ZK(2022)Key 008);the Wuhan Science and Technology Project(2020020601012259);the Guizhou Provincial Science and Technology Support Plan(qiankehezhicheng(2022)Key 026);the Key Cultivation Project of Guizhou University(201903)。

摘　　要：Nitrogen(N)fertilization is necessary for obtaining high rice yield.But excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental pollution.In this study,we assembled key genes involved in different nodes of N pathways to boost nitrate and ammonium uptake and assimilation,and to strengthen amino acid utilization to increase grain yield and nitrogen use efficiency(NUE)in rice.The combinations OsNPF8.9a×OsNR2,OsAMT1;2×OsGS1;2×OsAS1,and OsGS2×OsAS2×OsANT3 optimized nitrate assimilation,ammonium conversion,and N reutilization,respectively.In co-overexpressing rice lines obtained by co-transformation,the tiller number,biomass,and grain yield per plant of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of wild-type ZH11,the OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line,and the OsGS2×OsAS2×OsANT3-overexpressing line.The glutamine synthase activity,free amino acids,and nitrogen utilization efficiency(NUt E)of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of ZH11 and other lines that combined key genes.N influx efficiency was increased in the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line and OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line under a low ammonium and a low nitrate treatment,respectively.We propose that combining overexpression of OsAMT1;2,OsGS1;2,and OsAS1 is a promising breeding strategy for systematically increasing rice grain yield and NUE by focusing on key nodes in the N pathway.

关 键 词：Nitrogen Rice tillering Grain yield Nitrogen use efficiency CO-TRANSFORMATION 

分 类 号：S511[农业科学—作物学]