Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311  

作　　者：Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang 

机构地区：[1]Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College,Yangzhou University,Yangzhou 225009,Jiangsu,China [2]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China [3]School of Life Sciences,Nantong University,Nantong 226019,Jiangsu,China

出　　处：《The Crop Journal》2023年第3期887-894,共8页作物学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31771913,3207150123);the Jiangsu Key R&D Plan(BE2021301-1);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);the Open Funds of Jiangsu Key Laboratory of Crop Genetics and Physiology(NYCSL201904);the Open Funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding(PL202004)。

摘　　要：The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.

关 键 词：Rice WA-type CMS Restorer of fertility(Rf)gene Gene mapping 

分 类 号：S511.21[农业科学—作物学]