Effects of nitrogen fertilizer on protein synthesis, accumulation, and physicochemical properties in common buckwheat  

作　　者：Chenxi Wan Licheng Gao Jiale Wang Xinhui Lei Jincai Tao Baili Feng Jinfeng Gao 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China

出　　处：《The Crop Journal》2023年第3期941-950,共10页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2020YFD1000805-03);National Natural Science Foundation of China(31671631)。

摘　　要：Nitrogen(N)fertilization affects grain quality in common buckwheat(Fagopyrum esculentum Moench).But the effects of N fertilizer on various buckwheat protein parameters are not fully understood.This study aimed to investigate the synthesis,accumulation,and quality of buckwheat protein under four N application rates in the Loess Plateau,China.Optimal N application(180 kg N ha-1)improved yield,agronomic traits,and N transport and increased protein yield and protein component accumulation.Prolamin and glutelin accumulation first increased and then decreased with increasing N application.The relationships between the contents of protein components and the amount of applied N generally followed quadratic functions.Nitrate reductase and glutamine synthetase activities first increased and then decreased with increasing N levels.Optimal N fertilizer increased the waterholding capacity and thermal stability of buckwheat protein and reduced its emulsification capacity,but negligibly changed its oil-absorption capacity.Hydrophobic amino acids and glutelin content were the main factors affecting protein quality.

关 键 词：Common buckwheat Nitrogen fertilizer Storage protein Nitrogen metabolism Grain quality 

分 类 号：S517[农业科学—作物学]