Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay  

作　　者：Liang Dong Yanni Shi Pan Li Silin Zhong Yonghao Sun Fang Yang 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]State Key Laboratory of Agrobiotechnology,School of Life Sciences,The Chinese University of Hong Kong,Shatin 999077,Hong Kong,China

出　　处：《The Crop Journal》2023年第3期951-956,共6页作物学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(32172026);the 111 Project Crop Genomics and Molecular Breeding(B20051)。

摘　　要：Maize ear development determines the crop yield,and many important transcription factors(TFs)have been identified to function in this process.However,their transcriptional regulation mechanisms are still elusive.In this study,we generated the genome-wide DNA binding sites for 8 TFs which are known to function or highly expressed in inflorescence by applying the ts CUT&Tag method in maize leaf protoplast.We exposed a regulatory grid of 4 WUSCHEL-related homeobox(WOX)genes and revealed their potential regulatory mechanisms.In addition,a hierarchical regulation network for the determinacy and specification of maize inflorescence meristems were also constructed using the binding profiles of a floral development gene INDETERMINATE FLORAL APEX1(IFA1)and 3 MADS-box genes.Our study provides an in-depth understanding and new insights of the regulatory mechanisms during maize inflorescence development.

关 键 词：Maize inflorescence CUT&Tag Transcription factor WOX MADS-BOX 

分 类 号：S513[农业科学—作物学]