Construction of a high-density SSR genetic linkage map and identification of QTL for storage-root yield and dry-matter content in sweetpotato  

作　　者：Chenxing Zheng Zhicheng Jiang Yusha Meng Jun Yu Xinsun Yang Huan Zhang Ning Zhao Shaozhen He Shaopei Gao Hong Zhai Qingchang Liu 

机构地区：[1]Key Laboratory of Sweetpotato Biology and Biotechnology,Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis&Utilization,Ministry of Education,College of Agronomy&Biotechnology,China Agricultural University,Beijing 100193,China [2]Institute of Rural Development,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China [3]Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China

出　　处：《The Crop Journal》2023年第3期963-967,共5页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFD1001300,2019YFD1001301);the Earmarked Fund for CARS-10-Sweetpotato(CARS-10);the Beijing Food Crops Innovation Consortium Program(BAIC02-2022);Hebei Key R&D Program(20326320D,22322911D)。

摘　　要：Sweetpotato(Ipomoea batatas(L.)Lam.)is a widely grown food crop especially in developing countries.Increasing storage-root yield and dry-matter content has been the main breeding objective of the crop,and DNA marker-assisted breeding is needed for this purpose.In this study,using a mapping population of 500 F1 individuals from a cross between Xushu 18(female)and Xu 781(male),we constructed a highdensity genetic linkage map of sweetpotato using 601 simple-sequence repeat(SSR)primer pairs.The Xushu 18 map contained 90 linkage groups with 5547 SSR markers and spanned 18,263.5 cM,and the Xu 781 map contained 90 linkage groups with 4599 SSR markers and spanned 18,043.7 cM,representing the highest genome coverage yet reported for sweetpotato.We identified 33 QTL for storage-root yield and 16 QTL for dry-matter content,explaining respectively 6.5%–47.5%and 3.2%–18.9%of variation.These results provide a foundation for fine-mapping and cloning of QTL and for marker-assisted breeding in sweetpotato.

关 键 词：SWEETPOTATO SSR linkage map QTL Storage-root yield Dry-matter content 

分 类 号：S531[农业科学—作物学]