巨大芽孢杆菌应用于改善茄芯香气品质的研究  

Study on Aroma Quality Improvement of Cigar Filler Leaves by Bacillus megaterium

作　　者：毛亚浩 丁静怡 余君 陈雄 王志 杨春雷 姚兰 MAO Ya-hao;DING Jing-yi;YU Jun(College of Bioengineering and Food,Hubei University of Technology,Wuhan,Hubei 430068;Hubei Institute of Tobacco Science,Wuhan,Hubei 430030)

机构地区：[1]湖北工业大学生物工程与食品学院,湖北武汉430068 [2]湖北省烟草科学研究院,湖北武汉430030

出　　处：《安徽农业科学》2023年第10期10-15,78,共7页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31871789);中国烟草总公司重大专项项目(110202001039(XJ-01));湖北省烟草公司重点科技项目(027Y2019-003)。

摘　　要：为降低茄芯烟叶中木质素含量、改善烟叶品质,从烟叶提取液中筛选出一株具有木质素降解能力的菌株,鉴定为巨大芽孢杆菌(Bacillus megaterium)。其中木质素过氧化物酶活性达到19.03 U/L,锰过氧化物酶活性达到4.35 U/L。使用该菌株进行茄芯烟叶发酵,通过单因素试验及中心组合响应面法优化,得到最佳工艺条件:接种量7%、发酵温度37℃、起始含水量25%、发酵时间6 d。在此最佳条件下,茄芯烟叶木质素降解率达到23.02%,且发酵后中性香气总量较发酵前提高了30.22%。In order to reduce the lignin content in filler tobacco leaves and improve the quality of tobacco leaves,a strain with lignin-degrading ability was screened from tobacco leaf extract and identified as Bacillus megaterium.Among them,the enzymatic activity of lignin peroxidase reached 19.03 U/L,and the enzymatic activity of manganese peroxidase reached 4.35 U/L.The strain was used to ferment cigar tobacco leaves,and the optimal process conditions were obtained through single factor experiment and central combined response surface methodology optimization:the inoculum size was 7%,the fermentation temperature was 37℃,the initial water content was 25%,and the fermentation time was 6 d.Under this optimal condition,the degradation rate of lignin in cigar tobacco leaves reached 23.02%,and the total amount of neutral aroma after fermentation was increased by 30.22%compared with that before fermentation.

关 键 词：巨大芽孢杆菌 茄芯烟叶 木质素降解酶 响应面试验 中性香气 

分 类 号：Q815[生物学—生物工程]