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作 者:李珊珊 黄永芳[2] 董斌[3] 李荣喜[3] 赵平丽 张晖 LI Shan-shan;HUANG Yong-fang;DONG Bin(Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm South China Agricultural University,Guangzhou,Guangdong 510642;Guangdong AIB Polytechnic,Guangzhou,Guangdong 510507)
机构地区:[1]仲恺农业工程学院,广东广州510225 [2]华南农业大学广东省森林植物种质创新与利用重点实验室,广东广州510642 [3]广东农工商职业技术学院,广东广州510507 [4]鹤壁市淇滨区园林绿化中心,河南鹤壁458000
出 处:《安徽农业科学》2023年第10期85-88,共4页Journal of Anhui Agricultural Sciences
基 金:广东省林业科技创新项目(2017KJCX005)。
摘 要:[目的]筛选黎蒴扦插繁殖最优组合。[方法]以生长健壮的2年生黎蒴苗为试材,采用正交试验设计,分析不同激素、不同基质组分对扦插成活率、愈伤组织生长、芽生长率的影响,并筛选出最优组合。[结果]砂壤土利于插穗苗的扦插生长,插穗成活率为86.30%,2种激素处理对插穗苗扦插生长差异不大,NAA处理成活率为72.59%,IBA处理成活率为65.19%;砂壤土对愈伤组织生长的影响最大,插穗愈伤组织生长率为65.56%,2种激素处理对愈伤组织生长的影响无明显差异,NAA处理为50.74%,IBA处理为48.89%;黄心土为基质的芽生长率最高,为28.89%,2种激素处理对芽生长率无明显差异,NAA处理为25.56%,IBA处理为21.11%。[结论]扦插基质最优处理为砂壤土与CK的组合。[Objective]To select the optimal combination of cutting propagation of local tree Castanopsis fissa.[Method]The effects of different hormones and substrate components on the survival rate,callus growth and bud growth rate of cuttings were analyzed by orthogonal experiment.[Result]The results showed that sand loam was the best for cutting growth of cuttings,and the cuttings survival rate was 86.30%.There was little difference between the two hormone treatments on cutting growth of cuttings,the survival rate of NAA treatment was 72.59%,IBA treatment was 65.19%.The callus growth rate of cuttings was 65.56%in sandy loam soil.The callus growth rate of cuttings was 65.56%in sandy loam soil.The callus growth rate of cuttings was 50.74%in NAA treatment and 48.89%in IBA treatment.The shoot growth rate was the highest in yellow heart soil,which was 28.89%.There was no significant difference in the shoot growth rate between NAA and IBA treatments,which was 25.56%and 21.11%respectively.[Conclusion]The optimal cutting matrix combination was sandy loam and CK.
关 键 词:黎蒴 扦插繁殖 成活率 愈伤组织 NAA IBA
分 类 号:S723.1[农业科学—林木遗传育种]
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