Box-Behnken响应面法优化超声提取皱皮木瓜总黄酮工艺  

Optimization of Ultrasonic Extraction of Total Flavonoids from Chaenomeles speciosa by Box-Behnken Response Surface Methodology

作　　者：刘岩 吕宗凯 刘连芬[1] 钱关泽[1] LIU Yan;L Zong-kai;LIU Lian-fen(School of Life Science,Liaocheng University,Liaocheng,Shandong 252059)

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《安徽农业科学》2023年第10期144-148,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31070619,31170178);山东省自然科学基金项目(ZR2011CM045)。

摘　　要：以蔷薇科中的皱皮木瓜为研究对象,以其叶片为试验材料,分别进行料液比、提取时间、提取温度、乙醇浓度单因素试验探究总黄酮提取最适范围,通过响应面法(RSM)和Box-Behnken试验设计结合二次回流优化超声辅助提取总黄酮工艺。结果表明,不同因素对木瓜叶片总黄酮提取率的影响顺序为提取时间>乙醇浓度>料液比;最佳工艺条件为提取时间52 min、乙醇浓度74%、料液比1∶41(g∶mL)时,总黄酮为156.65 mg/g,提取率达到15.67%。Taking Chaenomeles speciosa in the Rosaceae as the research object and its leaves as the experimental material,the single factor experiments were conducted to explore the optimal range of total flavonoids extraction,including solid-liquid ratio,extraction time,extraction temperature and ethanol concentration.Response surface methodology(RSM)and Box-Behnken experimental design were combined with secondary reflux to optimize the ultrasonic assisted extraction process of total flavonoids.The results showed that the order of influence of different factors on the extraction rate of total flavonoids from papaya leaves was extraction time>ethanol concentration>solid-liquid ratio;the optimal process conditions were extraction time 52 minutes,ethanol concentration 74%and solid-liquid ratio 1∶41(g∶mL),the total flavonoids were 156.65 mg/g,and the extraction rate reached 15.67%.

关 键 词：皱皮木瓜 总黄酮 响应面法 提取工艺优化 

分 类 号：R284.2[医药卫生—中药学]