男性烟依赖者CDH23基因甲基化与颈动脉粥样硬化关系  

ASSOCIATION BETWEEN CDH23 GENE METHYLATION AND CAROTID ATHEROSCLEROSIS IN MALE TOBACCO-DEPEN-DENT PATIENTS

作　　者：彭琳 谭巧文 韩莹 赵静怡 郭宗君[1] PENG Lin;TAN Qiaowen;HAN Ying;ZHAO Jingyi;GUO Zongjun(Department of Geriatrics,Affiliated Hospital of Qingdao University,Qingdao 266100,China)

机构地区：[1]青岛大学附属医院老年医学科,山东青岛266100 [2]青岛滨海学院附属医院消化内科

出　　处：《青岛大学学报（医学版）》2023年第2期159-162,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(32071141);青岛市科技局基金资助项目(15-9-2-74-nsh)。

摘　　要：目的探讨男性烟依赖者CDH23基因位点甲基化改变及其与颈动脉粥样硬化发生的关系。方法选取青岛某三甲医院健康查体男性130例作为研究对象,有烟依赖史者64例纳入烟依赖组,无烟依赖史者66例纳入对照组。应用问卷调查方式搜集两组研究对象一般资料,包括年龄、受教育程度、既往疾病史、烟依赖史等;采用彩色多普勒超声仪器检测颈动脉内膜厚度;采集血样标本,检测CDH23基因位点甲基化数值;分析男性烟依赖者CDH23基因位点甲基化改变及其与颈动脉粥样硬化的关系。结果烟依赖组和对照组一般资料包括年龄、文化程度、既往疾病史比较,差异无统计学意义(P>0.05);烟依赖组CpG246、CpG48两个位点的甲基化程度低于对照组,差异有统计学意义(t=-2.454、-2.436,P<0.05);CpG246、CpG48位点甲基化水平与颈动脉内膜厚度相关(F=6.235、4.692,P<0.05),低甲基化水平时颈动脉内膜厚度显著大于高甲基化水平。结论烟依赖者CDH23基因CpG位点发生低甲基化改变,这种低甲基化改变与颈动脉粥样硬化发生密切相关。Objective To investigate the methylation of the CDH23 gene loci and its association with carotid atherosclerosis in male tobacco-dependent patients.Methods A total of 130 male subjects who underwent physical examination in a grade A tertiary hospital in Qingdao,China,were selected,among whom 64 with a history of tobacco dependence were enrolled as tobacco dependence group and 66 without such history were enrolled as control group.A questionnaire survey was performed to collect the general information of the two groups,including age,educational level,past history,and history of tobacco dependence.Color Doppler ultrasound was used to measure carotid intima thickness;blood samples were collected to measure the methylation value of the CDH23 gene loci;methylation of the CDH23 gene loci and its association with carotid atherosclerosis were analyzed in male tobacco-dependent patients.Results As for the general information,there were no significant differences in age,educational le-vel,and past history between the tobacco dependence group and the control group(P>0.05).The tobacco dependence group had a significantly lower methylation degree of CpG246 and CpG48 than the control group(t=-2.454,-2.436;P<0.05).The methylation levels of CpG246 and CpG48 loci were correlated with carotid intima thickness(F=6.235,4.692;P<0.05),and carotid intima thickness at a low methylation level was significantly larger than that at a high methylation level.Conclusion Hypomethylation is observed at the CpG locus of the CDH23 gene in tobacco-dependent patients,which is closely associated with the development of carotid atherosclerosis.

关 键 词：烟草对健康的危害 CDH23基因 甲基化 动脉粥样硬化 细胞黏附 

分 类 号：R543.5[医药卫生—心血管疾病] R163[医药卫生—内科学]