Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants  

作　　者：Pengcheng Wang Ping Hu Jinping Zhang Lixia Zhang Jing Zhang Zhengwang Zhang 

机构地区：[1]Jiangsu Key Laboratory for Biodiversity and Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing,210023,China [2]Taiyuan Zoo,Taiyuan,030009,China [3]Beijing Zoo Management Office,Beijing Key Laboratory of Captive Wildlife Technologies of Beijing Zoo,Beijing,100044,China [4]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,College of Life Sciences,Beijing Normal University,Beijing,100875,China

出　　处：《Avian Research》2023年第2期291-297,共7页鸟类学研究（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant No.31872244 to Z.Z.);Priority Academic Program Development of Jiangsu Higher Education Institutions,Beijing Zoo Management Office(No.zx2019016 to J.Z.);the Biodiversity Survey,Monitoring and Assessment Project(2019-2023)of the Ministry of Ecology and Environment,China(No.2019HB2096001006 to Z.Z.)。

摘　　要：Evaluating the genetic status of threatened species is an essential task in conservation genetics.However,the genetic status of threatened species has been mostly evaluated through techniques that fail to estimate genetic diversity at the whole genomic level.Next generation sequencing can meet this demand,but high quality samples such as blood or muscle tissues are required.However,it is difficult to collect such samples from threatened species because sampling work may impact their health.Therefore,it is essential to design a workflow to evaluate the whole genomic status of threatened species using non-destructive sampling.Even though non-destructive sampling has been used in traditional barcoding technique,the barcoding technique cannot evaluate the whole genomic status.Brown Eared Pheasant(Crossoptilon mantchuricum)is an endangered species,with captive populations maintained in Taiyuan Zoo,China,and Europe.However,the genetic diversity,inbreeding pattern,and mutation load of these two populations are unclear.To uncover the genetic status of these two captive populations,we applied 2b-RAD technology to evaluate the genomic status of these populations using feathers as samples.The feathers could be collected by non-destructive sampling.The results indicate that the Taiyuan Zoo population has a lower genetic diversity and higher inbreeding coefficient than the European population.The Taiyuan Zoo population has lethal mutations when homozygous.The current project uses a non-destructive sampling technique to evaluate the whole genomic status of the two captive populations,providing a paradigm for conservation genetics,which will facilitate the development of conservation biology.

关 键 词：Genetic diversity Inbreeding coefficient KINSHIP Mutation load Threatened species 

分 类 号：Q953[生物学—动物学]