DNA条形码技术在食用血制品掺杂成分鉴别中的应用  

Application of DNA barcoding technology in the identification of adulterated ingredients of edible blood products

作　　者：励炯[1] 吴琼 江海[1] 扈明洁 徐新怡 LI Jiong;WU Qiong;JIANG Hai;HU Ming-jie;XU Xin-yi(Hangzhou Institute for Food and Drug Control,Hangzhou,Zhejiang 310017,China;Zhejiang Fangyuan Test Group Co.,Ltd.,Hangzhou,Zhejiang 310017,China;Key Laboratory of Biosafety Detection for Zhejiang Market Regulation,Hangzhou,Zhejiang 310017,China)

机构地区：[1]杭州市食品药品检验研究院,浙江杭州310017 [2]浙江方圆检测集团股份有限公司,浙江杭州310017 [3]浙江省市场监管生物安全重点实验室,浙江杭州310017

出　　处：《食品与机械》2023年第5期43-48,138,共7页Food and Machinery

基　　金：国家市场监督管理总局科技计划项目(编号:2021MK141);浙江省市场监管生物安全重点实验室开放项目(编号:2023BS001)。

摘　　要：目的:建立一种可食用血制品中7种动物源成分(包括猪、牛、羊、鸡、鸭、鹅、兔)掺杂鉴别的分析方法。方法:血制品经DNA提取纯化及PCR扩增后,将克隆测序结果提交至7种血制品的DNA条形码本地数据库进行序列比对。对血制品的预处理方式、DNA提取方式以及PCR扩增条件进行选择和优化,并建立常见血制品掺杂模型,研究模型的最低掺杂检出率。结果:7种可食用血制品的DNA扩增效率为100%,各种掺杂模型中的最低掺杂检出率为5%;利用该法对25批次市售可食用血制品进行掺杂鉴定,发现有21批次存在掺有其他动物源成分的情况。结论:该方法简单、可靠,可作为日常可食用血制品质量监督的检测方法。Objective:A COI sequence-based full-length DNA barcoding technology was established to identify adulteration of seven animal-derived components(including pigs,cows,sheep,chickens,ducks,geese and rabbits)in edible blood products.Methods:After the blood products were extracted and purified by DNA extraction and PCR amplification,the clone sequencing results were submitted to the DNA barcode local database of the 7 blood products for sequence comparison.The pretreatment method,DNA extraction method and PCR amplification conditions of the blood products were also selected and optimized,and various common blood product adulteration models were established to study the lowest adulteration detection rate of the models.Results:The results showed that the DNA amplification efficiency of seven edible blood products was 100%,and the minimum adulteration detection rate in various adulteration models was 5%;25 batches of commercially available edible blood products were identified as adulterated using the method,and 21 batches were found to be adulterated with other animal-derived ingredients.Conclusion:The method is simple,reliable and can be used as a testing method for daily quality supervision of edible blood products.

关 键 词：DNA条形码 可食用血制品 COI 掺杂 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]