超声辅助酶法提取玉米皮中多糖及其抗氧化性测定  

Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity

作　　者：马丽媛[1] 黄雪莹 尚尔坤 郭丽[1] 张雅娜 MA Li-yuan;HUANG Xue-ying;SHANG Er-kun;GUO Li;ZHANG Ya-na(College of Food and Pharmaceutical Engineering,Suihua University,Suihua,Heilongjiang 152061,China;Suihua Inspection and Testing Center,Suihua,Heilongjiang 152000,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061 [2]绥化市检验检测中心,黑龙江绥化152000

出　　处：《食品与机械》2023年第5期186-192,202,共8页Food and Machinery

基　　金：绥化学院科研创新团队玉米功能性食品研发团队项目(编号:SIT05003);绥化学院社会服务工程项目(编号:SHU2022004)。

摘　　要：目的:利用超声波的超声震动作用和酶的酶解作用破坏细胞壁提高玉米皮多糖提取效率。方法:以多糖提取量为指标,在单因素试验基础上进行响应面试验优化超声辅助酶法提取玉米皮中多糖的工艺条件,并对玉米皮多糖的抗氧化性进行测定。结果:玉米皮多糖的最佳提取工艺为:复合酶比例(m纤维素酶∶m果胶酶)2∶1、酶用量2%(以玉米皮粉质量计)、酶解时间50 min、超声处理15 min、料液比(m玉米皮粉∶V蒸馏水)1∶15 (g/mL),此时提取的玉米皮多糖平均提取量为23.36μg/g。当玉米皮多糖样品质量浓度为0.2~1.0 mg/mL时,提取的玉米皮多糖具有一定的总还原能力、清除DPPH自由基和羟自由基能力,且随着样品质量浓度的增加而提高。结论:超声辅助酶法比单一酶法可提取出更多的多糖,且提取物能保持高效的抗氧化能力。Obiective:In the study,corn bran powder was used as raw material,and the cell wall was destroyed by ultrasonic vibration and enzymatic hydrolysis,so that corn bran polysaccharide could be dissolved more fully.Methods:The extracted amount of polysaccharide was taken as the index,response surface test optimization was carried out on the basis of single factor test.And the antioxidant activity of corn bran polysaccharide was determined.Results:The optimal extraction process of corn bran polysaccharide was obtained as follows:mass ratio of cellulase to pectinase 2∶1,enzyme dosage was 2%of the quality of corn bran powder,enzymatic hydrolysis time 50 min,ultrasonic treatment 15 min,and solid-liquid ratio(m corn bran powder∶V distilled water)1∶15(g/mL).At this time,the average amount of extracted corn bran polysaccharide was 23.36μg/g.The extracted corn bran polysaccharide had a certain total reducing capacity,scavenging DPPH free radical and hydroxyl radical ability,and it increased with the increase of mass concentration,when the mass concentration of corn bran polysaccharide sample was 0.2~1.0 mg/mL.Conclusion:Ultrasound-assisted enzymatic method could extract more polysaccharides than single enzymatic method,and the extract could maintain efficient antioxidant capacity.

关 键 词：玉米皮 多糖 超声辅助 酶法 抗氧化性 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]