基于UPLC特征图谱和一测多评法的穿心莲药材质量研究  

Study on Quality of Andrographis Herba Based on UPLC Fingerprint and Quantitative Analysis of Multi-components by Single Marker

作　　者：李振雨 赵书运 刘晓霞 何民友 冯涌微 位翠杰 张芳平 邓淙友 陈向东 孙冬梅 LI Zhenyu;ZHAO Shuyun;LIU Xiaoxia;HE Minyou;FENG Yongwei;WEI Cuijie;ZHANG Fangping;DENG Congyou;CHEN Xiangdong;SUN Dongmei(Guangdong E-fong Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《中国中医药信息杂志》2023年第6期141-147,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：中华人民共和国工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221)。

摘　　要：目的采用超高效液相色谱法(UPLC)建立穿心莲药材特征图谱及4个成分的一测多评方法。方法通过对12批穿心莲药材特征图谱的测定,建立穿心莲药材特征图谱共有模式,并进行相似度评价,经高分辨质谱分析和对照品比对,对特征峰进行指认。以穿心莲内酯为参照,建立新穿心莲内酯、14-去氧穿心莲内酯和脱水穿心莲内酯的一测多评法,并与外标法测定结果比较,判断方法的准确性。结果穿心莲药材特征图谱相似度为0.905~0.995,共确定12个特征峰,指认出其中7个。一测多评法与外标法测定结果的相对偏差在5.0%以内,表明该方法准确性良好。结论特征图谱结合一测多评法能够有效评价穿心莲药材的质量,该方法准确、可靠,系统适用性良好。Objective To establish the characteristic chromatogram of Andrographis Herba and quantitative analysis of multi-components by single marker(QAMS)of four components by ultra performance liquid chromatography(UPLC).Methods Through the determination of the specific chromatograms of 12 batches of Andrographis Herba,the common pattern of the specific chromatogram of Andrographis Herba was established and the similarity was evaluated.The characteristic peaks were identified by high resolution mass spectrometry analysis and comparison with reference materials.With andrographolide as a reference,quantitative analysis of QAMS was established for new neoandrographolide,14 deoxyandrographolide and dehydrated andrographolide and the results were compared with the external standard method(ESM)to determine the accuracy of the method.Results The similarity of the specific chromatograms of Andrographis Herba was 0.905–0.995,and 7 of the 12 characteristic peaks were identified.The relative deviation between QAMS and ESM was within 5.0%,indicating that the accuracy of the method was good.Conclusion The specific chromatogram combined with QAMS can effectively evaluate the quality of Andrographis Herba,and the method is accurate,reliable and has good system applicability.

关 键 词：穿心莲 特征图谱 一测多评法 质量研究 

分 类 号：R282.6[医药卫生—中药学]