The spliceophilin CYP18-2 is mainly involved in the splicing of retained introns under heat stress in Arabidopsis  

作　　者：Areum Lee Hyun Ji Park Seung Hee Jo Haemyeong Jung Hyun-Soon Kim Hyo-Jun Lee Youn-Sung Kim Choonkyun Jung Hye Sun Cho 

机构地区：[1]Plant Systems Engineering Research Center,Korea Research Institute of Bioscience and Biotechnology(KRIBB),Daejeon 34141,Korea [2]Department of Biosystems and Bioengineering,KRIBB School of Biotechnology,University of Science and Technology(UST),Daejeon 34141,Korea [3]Department of Functional Genomics,KRIBB School of Bioscience,UST,Daejeon 34113,Korea [4]Department of Biotechnology,NongWoo Bio,Anseong 17558,Korea [5]Department of International Agricultural Technology and Crop Biotechnology Institute/Green Bio Science and Technology,Seoul National University,Pyeongchang 25354,Korea [6]Department of Agriculture,Forestry,and Bioresources and Integrated Major in Global Smart Farm,College of Agriculture and Life Sciences,Seoul National University,Seoul 08826,Korea

出　　处：《Journal of Integrative Plant Biology》2023年第5期1113-1133,共21页植物学报（英文版）

基　　金：funded by the New Breeding Technology program(no,PJ01686202);the National Research Foundation of Korea(NRF;No.2022R1A2B5B02002008);Research Initiative Programs of the Korea Research Institute of Bioscience and Biotechnology(KRIBB;No.5372322)grants to H.C.

摘　　要：Peptidyl-prolyl isomerase-like 1(PPIL1)is associated with the human spliceosome complex.However,its function in pre-mRNA splicing remains unclear.In this study,we show that Arabidopsis thaliana CYCLOPHILIN 18-2(AtCYP18-2),a PPIL1 homolog,plays an essential role in heat tolerance by regulating pre-mRNA splicing.Under heat stress conditions,AtCYP18-2 expression was upregulated in mature plants and GFP-tagged AtCYP18-2 redistributed to nuclear and cytoplasmic puncta.We determined that AtCYP18-2 interacts with several spliceosome complex B^(ACT)components in nuclear puncta and is primarily associated with the small nuclear RNAs U5 and U6 in response to heat stress.The AtCYP18-2 loss-of-function allele cyp18-2 engineered by CRISPR/Cas9-mediated gene editing exhibited a hypersensitive phenotype to heat stress relative to the wild type.Moreover,global transcriptome profiling showed that the cyp18-2 mutation affects alternative splicing of heat stress–responsive genes under heat stress conditions,particularly intron retention(IR).The abundance of most intron-containing transcripts of a subset of genes essential for thermotolerance decreased in cyp18-2 compared to the wild type.Furthermore,the intron-containing transcripts of two heat stress-related genes,HEAT SHOCK PROTEIN 101(HSP101)and HEAT SHOCK FACTOR A2(HSFA2),produced functional proteins.HSP101-IR-GFP localization was responsive to heat stress,and HSFA2-Ⅲ-IR interacted with HSF1 and HSP90.1 in plant cells.Our findings reveal that CYP18-2 functions as a splicing factor within the B~(ACT)spliceosome complex and is crucial for ensuring the production of adequate levels of alternatively spliced transcripts to enhance thermotolerance.

关 键 词：alternative splicing CYP18-2 HSFA2-Ⅲ intron retention spliceophilin spliceosome complex B~(ACT) THERMOTOLERANCE 

分 类 号：Q943.2[生物学—植物学]