Heteromerization of short-chain trans-prenyltransferase controls precursor allocation within a plastidial terpenoid network  

作　　者：Yihua Ma Qingwen Chen Yaoyao Wang Fengxia Zhang Chengyuan Wang Guodong Wang 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]UniversityofChineseAcademyof Sciences,Beijing100039,China [3]Center for Microbes,Development and Health,Institut Pasteur of Shanghai,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Journal of Integrative Plant Biology》2023年第5期1170-1182,共13页植物学报（英文版）

基　　金：financially supported by the National Key R&D Program of China(2018YFA0900600);the National Natural Science Foundation of China(31970315);the Key R&D Program of Shandong Province(2019JZZY020610);the State Key Laboratory of Plant Genomics of China(grant no.SKLPG2016A-13)。

摘　　要：Terpenes are the largest and most diverse class of plant specialized metabolites.Sesterterpenes(C25),which are derived from the plastid methylerythritol phosphate pathway,were recently characterized in plants.In Arabidopsis thaliana,four genes encoding geranylfarnesyl diphosphate synthase(GFPPS)(AtGFPPS1 to 4)are responsible for the production of GFPP,which is the common precursor for sesterterpene biosynthesis.However,the interplay between sesterterpenes and other known terpenes remain elusive.Here,we first provide genetic evidence to demonstrate that GFPPSs are responsible for sesterterpene production in Arabidopsis.Blockage of the sesterterpene pathway at the GFPPS step increased the production of geranylgeranyl diphosphate(GGPP)-derived terpenes.Interestingly,co-expression of sester TPSs in GFPPSOE(overexpression)plants rescued the phenotypic changes of GFPPS-OE plants by restoring the endogenous GGPP.We further demonstrated that,in addition to precursor(DMAPP/IPP)competition by GFPPS and GGPP synthase(GGPPS)in plastids,GFPPS directly decreased the activity of GGPPS through protein-protein interaction,ultimately leading to GGPP deficiency in planta.Our study provides a new regulatory mechanism of the plastidial terpenoid network in plant cells.

关 键 词：Arabidopsis geranylfarnesyl diphosphate synthase geranylgeranyl pyrophosphate synthase PLASTID sesterterpene terpene network 

分 类 号：Q945[生物学—植物学]